TY - CHAP
T1 - Renal sympathetic denervation in rats
AU - Eriguchi, Masahiro
AU - Tsuruya, Kazuhiko
PY - 2016
Y1 - 2016
N2 - Experimental renal sympathetic denervation is a well-established technique. Classically, renal sympathetic denervation is achieved by dorsal rhizotomy. While more recently, direct renal sympathetic denervation is typically applied by stripping all visible renal nerve bundles followed by painting with a solution of 10 % phenol in ethanol to remove the remaining nerves. In clinical settings, a reliable marker of renal sympathetic denervation or renal sympathetic overactivity has not been established. However, in experimental models, successful renal sympathetic denervation is validated by a decrease in renal norepinephrine content levels. This facilitates the assessment of incomplete denervation by technical failure and reinnervation for long-term experimental models. In this chapter, we introduce comprehensive methods for direct renal sympathetic denervation and measurement of renal norepinephrine content levels.
AB - Experimental renal sympathetic denervation is a well-established technique. Classically, renal sympathetic denervation is achieved by dorsal rhizotomy. While more recently, direct renal sympathetic denervation is typically applied by stripping all visible renal nerve bundles followed by painting with a solution of 10 % phenol in ethanol to remove the remaining nerves. In clinical settings, a reliable marker of renal sympathetic denervation or renal sympathetic overactivity has not been established. However, in experimental models, successful renal sympathetic denervation is validated by a decrease in renal norepinephrine content levels. This facilitates the assessment of incomplete denervation by technical failure and reinnervation for long-term experimental models. In this chapter, we introduce comprehensive methods for direct renal sympathetic denervation and measurement of renal norepinephrine content levels.
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U2 - 10.1007/978-1-4939-3353-2_5
DO - 10.1007/978-1-4939-3353-2_5
M3 - Chapter
C2 - 26676126
AN - SCOPUS:84950286625
T3 - Methods in Molecular Biology
SP - 45
EP - 52
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -