Repetitive tensile stress to rat caudal vertebrae inducing cartilage formation in the spinal ligaments: A possible role of mechanical stress in the development of ossification of the spinal ligaments

Nobuaki Tsukamoto, Takeshi Maeda, Hiromasa Miura, Seiya Jingushi, Akira Hosokawa, Katsumi Harimaya, Hidehiko Higaki, Kousaku Kurata, Yukihide Iwamoto

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Object. Mechanical stress has been considered one of the important factors in ossification of the spinal ligaments. According to previous clinical and in vitro studies, the accumulation of tensile stress to these ligaments may be responsible for ligament ossification. To elucidate the relationship between such mechanical stress and the development of ossification of the spinal ligaments, the authors established an animal experimental model in which the in vivo response of the spinal ligaments to direct repetitive tensile loading could be observed. Methods. The caudal vertebrae of adult Wistar rats were studied. After creating a novel stimulating apparatus, cyclic tensile force was loaded to rat caudal spinal ligaments at 10 N in 600 to 1800 cycles per day for up to 2 weeks. The morphological responses were then evaluated histologically and immunohistochemically. After the loadings, ectopic cartilaginous formations surrounded by proliferating round cells were observed near the insertion of the spinal ligaments. Several areas of the cartilaginous tissue were accompanied by woven bone. Bone morphogenetic protein-2 expression was clearly observed in the cytoplasm of the proliferating round cells. The histological features of the rat spinal ligaments induced by the tensile loadings resembled those of spinal ligament ossification observed in humans. Conclusions. The findings obtained in the present study strongly suggest that repetitive tensile stress to the spinal ligaments is one of the important causes of ligament ossification in the spine.

Original languageEnglish
Pages (from-to)234-242
Number of pages9
JournalJournal of Neurosurgery: Spine
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 1 2006
Externally publishedYes

Fingerprint

Mechanical Stress
Ligaments
Osteogenesis
Cartilage
Spine
Bone Morphogenetic Protein 2
Wistar Rats
Cytoplasm
Animal Models

All Science Journal Classification (ASJC) codes

  • Surgery
  • Neurology
  • Clinical Neurology

Cite this

Repetitive tensile stress to rat caudal vertebrae inducing cartilage formation in the spinal ligaments : A possible role of mechanical stress in the development of ossification of the spinal ligaments. / Tsukamoto, Nobuaki; Maeda, Takeshi; Miura, Hiromasa; Jingushi, Seiya; Hosokawa, Akira; Harimaya, Katsumi; Higaki, Hidehiko; Kurata, Kousaku; Iwamoto, Yukihide.

In: Journal of Neurosurgery: Spine, Vol. 5, No. 3, 01.09.2006, p. 234-242.

Research output: Contribution to journalArticle

@article{c587318f3c9940798032a05ebd9a1bd6,
title = "Repetitive tensile stress to rat caudal vertebrae inducing cartilage formation in the spinal ligaments: A possible role of mechanical stress in the development of ossification of the spinal ligaments",
abstract = "Object. Mechanical stress has been considered one of the important factors in ossification of the spinal ligaments. According to previous clinical and in vitro studies, the accumulation of tensile stress to these ligaments may be responsible for ligament ossification. To elucidate the relationship between such mechanical stress and the development of ossification of the spinal ligaments, the authors established an animal experimental model in which the in vivo response of the spinal ligaments to direct repetitive tensile loading could be observed. Methods. The caudal vertebrae of adult Wistar rats were studied. After creating a novel stimulating apparatus, cyclic tensile force was loaded to rat caudal spinal ligaments at 10 N in 600 to 1800 cycles per day for up to 2 weeks. The morphological responses were then evaluated histologically and immunohistochemically. After the loadings, ectopic cartilaginous formations surrounded by proliferating round cells were observed near the insertion of the spinal ligaments. Several areas of the cartilaginous tissue were accompanied by woven bone. Bone morphogenetic protein-2 expression was clearly observed in the cytoplasm of the proliferating round cells. The histological features of the rat spinal ligaments induced by the tensile loadings resembled those of spinal ligament ossification observed in humans. Conclusions. The findings obtained in the present study strongly suggest that repetitive tensile stress to the spinal ligaments is one of the important causes of ligament ossification in the spine.",
author = "Nobuaki Tsukamoto and Takeshi Maeda and Hiromasa Miura and Seiya Jingushi and Akira Hosokawa and Katsumi Harimaya and Hidehiko Higaki and Kousaku Kurata and Yukihide Iwamoto",
year = "2006",
month = "9",
day = "1",
doi = "10.3171/spi.2006.5.3.234",
language = "English",
volume = "5",
pages = "234--242",
journal = "Journal of Neurosurgery: Spine",
issn = "1547-5654",
publisher = "American Association of Neurological Surgeons",
number = "3",

}

TY - JOUR

T1 - Repetitive tensile stress to rat caudal vertebrae inducing cartilage formation in the spinal ligaments

T2 - A possible role of mechanical stress in the development of ossification of the spinal ligaments

AU - Tsukamoto, Nobuaki

AU - Maeda, Takeshi

AU - Miura, Hiromasa

AU - Jingushi, Seiya

AU - Hosokawa, Akira

AU - Harimaya, Katsumi

AU - Higaki, Hidehiko

AU - Kurata, Kousaku

AU - Iwamoto, Yukihide

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Object. Mechanical stress has been considered one of the important factors in ossification of the spinal ligaments. According to previous clinical and in vitro studies, the accumulation of tensile stress to these ligaments may be responsible for ligament ossification. To elucidate the relationship between such mechanical stress and the development of ossification of the spinal ligaments, the authors established an animal experimental model in which the in vivo response of the spinal ligaments to direct repetitive tensile loading could be observed. Methods. The caudal vertebrae of adult Wistar rats were studied. After creating a novel stimulating apparatus, cyclic tensile force was loaded to rat caudal spinal ligaments at 10 N in 600 to 1800 cycles per day for up to 2 weeks. The morphological responses were then evaluated histologically and immunohistochemically. After the loadings, ectopic cartilaginous formations surrounded by proliferating round cells were observed near the insertion of the spinal ligaments. Several areas of the cartilaginous tissue were accompanied by woven bone. Bone morphogenetic protein-2 expression was clearly observed in the cytoplasm of the proliferating round cells. The histological features of the rat spinal ligaments induced by the tensile loadings resembled those of spinal ligament ossification observed in humans. Conclusions. The findings obtained in the present study strongly suggest that repetitive tensile stress to the spinal ligaments is one of the important causes of ligament ossification in the spine.

AB - Object. Mechanical stress has been considered one of the important factors in ossification of the spinal ligaments. According to previous clinical and in vitro studies, the accumulation of tensile stress to these ligaments may be responsible for ligament ossification. To elucidate the relationship between such mechanical stress and the development of ossification of the spinal ligaments, the authors established an animal experimental model in which the in vivo response of the spinal ligaments to direct repetitive tensile loading could be observed. Methods. The caudal vertebrae of adult Wistar rats were studied. After creating a novel stimulating apparatus, cyclic tensile force was loaded to rat caudal spinal ligaments at 10 N in 600 to 1800 cycles per day for up to 2 weeks. The morphological responses were then evaluated histologically and immunohistochemically. After the loadings, ectopic cartilaginous formations surrounded by proliferating round cells were observed near the insertion of the spinal ligaments. Several areas of the cartilaginous tissue were accompanied by woven bone. Bone morphogenetic protein-2 expression was clearly observed in the cytoplasm of the proliferating round cells. The histological features of the rat spinal ligaments induced by the tensile loadings resembled those of spinal ligament ossification observed in humans. Conclusions. The findings obtained in the present study strongly suggest that repetitive tensile stress to the spinal ligaments is one of the important causes of ligament ossification in the spine.

UR - http://www.scopus.com/inward/record.url?scp=33749048051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749048051&partnerID=8YFLogxK

U2 - 10.3171/spi.2006.5.3.234

DO - 10.3171/spi.2006.5.3.234

M3 - Article

C2 - 16961085

AN - SCOPUS:33749048051

VL - 5

SP - 234

EP - 242

JO - Journal of Neurosurgery: Spine

JF - Journal of Neurosurgery: Spine

SN - 1547-5654

IS - 3

ER -