Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli

Kenji Kurokawa, Satoshi Nishida, Akiko Emoto, Kazuhisa Sekimizu, Tsutomu Katayama

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The ATP-bound but not the ADP-bound form of DnaA protein is active for replication initiation at the Escherichia coli chromosomal origin. The hydrolysis of ATP bound to DnaA is accelerated by the sliding clamp of DNA polymerase III loaded on DNA. Using a culture of randomly dividing cells, we now have evidence that the cellular level of ATP-DnaA is repressed to only ~ 20% of the total DnaA molecules, in a manner depending on DNA replication. In a synchronized culture, the ATP-DnaA level showed oscillation that has a temporal increase around the time of initiation, and decreases rapidly after initiation. Production of ATP-DnaA depended on concomitant protein synthesis, but not on SOS response, Dam or SeqA. Regeneration of ATP-DnaA from ADP-DnaA was also observed. These results indicate that the nucleotide form shifts of DnaA are tightly linked with an epistatic cell cycle event and with the chromosomal replication system.

Original languageEnglish
Pages (from-to)6642-6652
Number of pages11
JournalEMBO Journal
Volume18
Issue number23
Publication statusPublished - Dec 1 1999

Fingerprint

Adenine Nucleotides
Escherichia coli Proteins
Escherichia coli
Adenosine Triphosphate
Proteins
Adenosine Diphosphate
DNA Polymerase III
DNA
Clamping devices
DNA Replication
Dams
Regeneration
Hydrolysis
Cell Cycle
Nucleotides
Cells
Molecules

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli. / Kurokawa, Kenji; Nishida, Satoshi; Emoto, Akiko; Sekimizu, Kazuhisa; Katayama, Tsutomu.

In: EMBO Journal, Vol. 18, No. 23, 01.12.1999, p. 6642-6652.

Research output: Contribution to journalArticle

Kurokawa, Kenji ; Nishida, Satoshi ; Emoto, Akiko ; Sekimizu, Kazuhisa ; Katayama, Tsutomu. / Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli. In: EMBO Journal. 1999 ; Vol. 18, No. 23. pp. 6642-6652.
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