Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand

Takehiro Yasukawa, Aurelio Reyes, Tricia J. Cluett, Ming Yao Yang, Mark Bowmaker, Howard T. Jacobs, Ian J. Holt

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146 Citations (Scopus)


Using two-dimensional agarose gel electrophoresis, we show that mitochondrial DNA (mtDNA) replication of birds and mammals frequently entails ribonucleotide incorporation throughout the lagging strand (RITOLS). Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5′ ends of DNA, initiation of RITOLS replication occurs in the major non-coding region of vertebrate mtDNA and is effectively unidirectional. In some cases, conversion of nascent RNA strands to DNA starts at defined loci, the most prominent of which maps, in mammalian mtDNA, in the vicinity of the site known as the light-strand origin.

Original languageEnglish
Pages (from-to)5358-5371
Number of pages14
JournalEMBO Journal
Issue number22
Publication statusPublished - Nov 15 2006


All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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