Repression of Fat-Dependent Intestinal Apo A-IV mRNA Abundance by Medium Chain Triacylglycerols and Proteins, and Elevation by Carbohydrates of Fat-Dependent Apo A-IV Transport in Suckling Rat Pups

Masao Sato, Koji Nagao, Tadao Hayami, Katsumi Imaizumi, Michihiro Sugano

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5 Citations (Scopus)

Abstract

Nutrients regulating intestinal apo A-IV synthesis and secretion in developing rats have not been clearly defined. We examined the effect of ingestion of fats, carbohydrates and proteins on the abundance of intestinal apo A-IV mRNA and the serum concentration of apo A-IV in 14-day-old suckling rat pups fasted overnight. In pups ingesting long-chain fatty acid-fat (soybean oil, triolein: LCT), there was a prompt elevation of the mRNA at 1.5 h after ingestion, although a graded dose of soybean oil did not result in a comparable elevation of the apo A-IV mRNA. In pups on MCT, but not trilaurin, there was a repression of the LCT-dependent elevation of the message. In pups on Intralipid (composed of soybean oil, lecithin and glycerol), mRNA was elevated at 6 h after ingestion. Administration of Intralipid with lactose, glucose, fructose and sucrose induced a rapid elevation of mRNA together with elevation of serum apo A-IV, although administration of casein, whey proteins and soybean proteins resulted in repression of the Intralipid-dependent mRNA elevation. The message correlated weakly to the serum apo A-IV and triacylglycerols and with no correlation to intestinal fat accumulation. These results suggest that metabolic events following the ingestion of milk components modulate intestinal apo A-IV expression in developing rats, possibly through mucosal fatty acid utilization.

Original languageEnglish
Pages (from-to)293-306
Number of pages14
JournalJournal of Nutritional Science and Vitaminology
Volume41
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

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Triglycerides
Fats
Carbohydrates
Messenger RNA
Soybean Oil
Eating
Proteins
Fatty Acids
Serum
Triolein
Soybean Proteins
Lecithins
Lactose
Caseins
apolipoprotein A-IV
Fructose
Glycerol
Sucrose
Milk
Glucose

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

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title = "Repression of Fat-Dependent Intestinal Apo A-IV mRNA Abundance by Medium Chain Triacylglycerols and Proteins, and Elevation by Carbohydrates of Fat-Dependent Apo A-IV Transport in Suckling Rat Pups",
abstract = "Nutrients regulating intestinal apo A-IV synthesis and secretion in developing rats have not been clearly defined. We examined the effect of ingestion of fats, carbohydrates and proteins on the abundance of intestinal apo A-IV mRNA and the serum concentration of apo A-IV in 14-day-old suckling rat pups fasted overnight. In pups ingesting long-chain fatty acid-fat (soybean oil, triolein: LCT), there was a prompt elevation of the mRNA at 1.5 h after ingestion, although a graded dose of soybean oil did not result in a comparable elevation of the apo A-IV mRNA. In pups on MCT, but not trilaurin, there was a repression of the LCT-dependent elevation of the message. In pups on Intralipid (composed of soybean oil, lecithin and glycerol), mRNA was elevated at 6 h after ingestion. Administration of Intralipid with lactose, glucose, fructose and sucrose induced a rapid elevation of mRNA together with elevation of serum apo A-IV, although administration of casein, whey proteins and soybean proteins resulted in repression of the Intralipid-dependent mRNA elevation. The message correlated weakly to the serum apo A-IV and triacylglycerols and with no correlation to intestinal fat accumulation. These results suggest that metabolic events following the ingestion of milk components modulate intestinal apo A-IV expression in developing rats, possibly through mucosal fatty acid utilization.",
author = "Masao Sato and Koji Nagao and Tadao Hayami and Katsumi Imaizumi and Michihiro Sugano",
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T1 - Repression of Fat-Dependent Intestinal Apo A-IV mRNA Abundance by Medium Chain Triacylglycerols and Proteins, and Elevation by Carbohydrates of Fat-Dependent Apo A-IV Transport in Suckling Rat Pups

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AU - Nagao, Koji

AU - Hayami, Tadao

AU - Imaizumi, Katsumi

AU - Sugano, Michihiro

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AB - Nutrients regulating intestinal apo A-IV synthesis and secretion in developing rats have not been clearly defined. We examined the effect of ingestion of fats, carbohydrates and proteins on the abundance of intestinal apo A-IV mRNA and the serum concentration of apo A-IV in 14-day-old suckling rat pups fasted overnight. In pups ingesting long-chain fatty acid-fat (soybean oil, triolein: LCT), there was a prompt elevation of the mRNA at 1.5 h after ingestion, although a graded dose of soybean oil did not result in a comparable elevation of the apo A-IV mRNA. In pups on MCT, but not trilaurin, there was a repression of the LCT-dependent elevation of the message. In pups on Intralipid (composed of soybean oil, lecithin and glycerol), mRNA was elevated at 6 h after ingestion. Administration of Intralipid with lactose, glucose, fructose and sucrose induced a rapid elevation of mRNA together with elevation of serum apo A-IV, although administration of casein, whey proteins and soybean proteins resulted in repression of the Intralipid-dependent mRNA elevation. The message correlated weakly to the serum apo A-IV and triacylglycerols and with no correlation to intestinal fat accumulation. These results suggest that metabolic events following the ingestion of milk components modulate intestinal apo A-IV expression in developing rats, possibly through mucosal fatty acid utilization.

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