Reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of crops in a nematode-resistant Japanese black pine seed orchard

S. Goto, Atsushi Watanabe, F. Miyahara, Y. Mori

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14 Citations (Scopus)

Abstract

The reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of orchard crops were evaluated, using five pairs of microsatellite markers, in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones. The paternity of each open-pollinated seed was determined by comparing the genotypes of seeds from six clones (24 trees) with genotypes of the 16 orchard clones and two trees (N1, N2) representing other genotypes that had been inadvertently included in the orchard. Out of 384 seeds examined, the paternity of 316 seeds (82.3%) was assigned to the clones within the seed orchard. On average, the male reproductive success of orchard clones varied from 0.0% to 10.5%, and was significantly related to the male-flowering fecundity of each clone. It was not related to the synchrony of flowering phenology between mates. The expected proportions of seeds produced by clonal trees as a result of pollination by orchard clones, and by contaminating pollen originating from internal and external sources were estimated at 86.8%, 3.3% and 9.9%, respectively. Nematode-resistant seedlings of Japanese black pine were produced from surviving 2-yr seedlings that had previously been inoculated with pinewood nematode (Bursaphelenchus xylophilus). Without pollen contamination, the survival rate of seedlings produced by mating between resistant clones is expected to be 62.4%. However, in this orchard the figure was reduced to 57.5%, due to pollen contamination from both internal and external sources.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalSilvae Genetica
Volume54
Issue number2
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Pinus thunbergii
seed orchards
Pollen
orchard
reproductive success
nematode
clone
Seeds
pollen
Clone Cells
Nematoda
clones
seed
crop
orchards
crops
Seedlings
Bursaphelenchus xylophilus
seeds
genotype

All Science Journal Classification (ASJC) codes

  • Forestry
  • Plant Science
  • Genetics

Cite this

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title = "Reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of crops in a nematode-resistant Japanese black pine seed orchard",
abstract = "The reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of orchard crops were evaluated, using five pairs of microsatellite markers, in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones. The paternity of each open-pollinated seed was determined by comparing the genotypes of seeds from six clones (24 trees) with genotypes of the 16 orchard clones and two trees (N1, N2) representing other genotypes that had been inadvertently included in the orchard. Out of 384 seeds examined, the paternity of 316 seeds (82.3{\%}) was assigned to the clones within the seed orchard. On average, the male reproductive success of orchard clones varied from 0.0{\%} to 10.5{\%}, and was significantly related to the male-flowering fecundity of each clone. It was not related to the synchrony of flowering phenology between mates. The expected proportions of seeds produced by clonal trees as a result of pollination by orchard clones, and by contaminating pollen originating from internal and external sources were estimated at 86.8{\%}, 3.3{\%} and 9.9{\%}, respectively. Nematode-resistant seedlings of Japanese black pine were produced from surviving 2-yr seedlings that had previously been inoculated with pinewood nematode (Bursaphelenchus xylophilus). Without pollen contamination, the survival rate of seedlings produced by mating between resistant clones is expected to be 62.4{\%}. However, in this orchard the figure was reduced to 57.5{\%}, due to pollen contamination from both internal and external sources.",
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T1 - Reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of crops in a nematode-resistant Japanese black pine seed orchard

AU - Goto, S.

AU - Watanabe, Atsushi

AU - Miyahara, F.

AU - Mori, Y.

PY - 2005

Y1 - 2005

N2 - The reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of orchard crops were evaluated, using five pairs of microsatellite markers, in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones. The paternity of each open-pollinated seed was determined by comparing the genotypes of seeds from six clones (24 trees) with genotypes of the 16 orchard clones and two trees (N1, N2) representing other genotypes that had been inadvertently included in the orchard. Out of 384 seeds examined, the paternity of 316 seeds (82.3%) was assigned to the clones within the seed orchard. On average, the male reproductive success of orchard clones varied from 0.0% to 10.5%, and was significantly related to the male-flowering fecundity of each clone. It was not related to the synchrony of flowering phenology between mates. The expected proportions of seeds produced by clonal trees as a result of pollination by orchard clones, and by contaminating pollen originating from internal and external sources were estimated at 86.8%, 3.3% and 9.9%, respectively. Nematode-resistant seedlings of Japanese black pine were produced from surviving 2-yr seedlings that had previously been inoculated with pinewood nematode (Bursaphelenchus xylophilus). Without pollen contamination, the survival rate of seedlings produced by mating between resistant clones is expected to be 62.4%. However, in this orchard the figure was reduced to 57.5%, due to pollen contamination from both internal and external sources.

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