Reprogramming of mouse and human cells to pluripotency using mature microRNAs

Norikatsu Miyoshi, Hideshi Ishii, Hiroaki Nagano, Naotsugu Haraguchi, Dyah Laksmi Dewi, Yoshihiro Kano, Shinpei Nishikawa, Masahiro Tanemura, Koshi Mimori, Fumiaki Tanaka, Toshiyuki Saito, Junichi Nishimura, Ichiro Takemasa, Tsunekazu Mizushima, Masataka Ikeda, Hirofumi Yamamoto, Mitsugu Sekimoto, Yuichiro Doki, Masaki Mori

Research output: Contribution to journalArticlepeer-review

546 Citations (Scopus)

Abstract

Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.

Original languageEnglish
Pages (from-to)633-638
Number of pages6
JournalCell stem cell
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 3 2011

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

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