TY - JOUR
T1 - Reprogramming of mouse and human cells to pluripotency using mature microRNAs
AU - Miyoshi, Norikatsu
AU - Ishii, Hideshi
AU - Nagano, Hiroaki
AU - Haraguchi, Naotsugu
AU - Dewi, Dyah Laksmi
AU - Kano, Yoshihiro
AU - Nishikawa, Shinpei
AU - Tanemura, Masahiro
AU - Mimori, Koshi
AU - Tanaka, Fumiaki
AU - Saito, Toshiyuki
AU - Nishimura, Junichi
AU - Takemasa, Ichiro
AU - Mizushima, Tsunekazu
AU - Ikeda, Masataka
AU - Yamamoto, Hirofumi
AU - Sekimoto, Mitsugu
AU - Doki, Yuichiro
AU - Mori, Masaki
N1 - Funding Information:
We would like to thank Dr. S. Yamanaka (Kyoto University) for fruitful discussions. We thank Drs. J.H. Moon, S. Miyazaki, H. Hoshnino, and Y. Omura for fruitful discussions and K. Kitagawa and S. Yamane for special technical assistance. This work was partly supported by a grant from Core Research for Evolutional Science and Technology (CREST) (M.M. and K.M.), a Grant-in-Aid for Scientific Research on Innovative Areas (M.M.), Grants-in-Aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology (S: 21229015) (M.M.), Grants-in-Aid for the 3rd Comprehensive 10-year Strategy for Cancer Control from the Ministry of Health, Labour and Welfare (M.M., H.I.), the Funding Program for Next Generation World-Leading Researchers, Japan (LS094) (K.M.), and a Grant-in-Aid from the Tokyo Biochemical Research Foundation (M.M.). The authors declare no competing financial interests.
PY - 2011/6/3
Y1 - 2011/6/3
N2 - Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.
AB - Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.
UR - http://www.scopus.com/inward/record.url?scp=79957855262&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957855262&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2011.05.001
DO - 10.1016/j.stem.2011.05.001
M3 - Article
C2 - 21620789
AN - SCOPUS:79957855262
SN - 1934-5909
VL - 8
SP - 633
EP - 638
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 6
ER -
