Reprogramming of mouse and human cells to pluripotency using mature microRNAs

Norikatsu Miyoshi, Hideshi Ishii, Hiroaki Nagano, Naotsugu Haraguchi, Dyah Laksmi Dewi, Yoshihiro Kano, Shinpei Nishikawa, Masahiro Tanemura, Koshi Mimori, Fumiaki Tanaka, Toshiyuki Saito, Junichi Nishimura, Ichiro Takemasa, Tsunekazu Mizushima, Masataka Ikeda, Hirofumi Yamamoto, Mitsugu Sekimoto, Yuichiro Doki, Masaki Mori

Research output: Contribution to journalArticle

516 Citations (Scopus)

Abstract

Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.

Original languageEnglish
Pages (from-to)633-638
Number of pages6
JournalCell stem cell
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 3 2011

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MicroRNAs
Chemical Phenomena
Induced Pluripotent Stem Cells
Regenerative Medicine
Transfection
Biomedical Research
Transcription Factors
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

Cite this

Miyoshi, N., Ishii, H., Nagano, H., Haraguchi, N., Dewi, D. L., Kano, Y., ... Mori, M. (2011). Reprogramming of mouse and human cells to pluripotency using mature microRNAs. Cell stem cell, 8(6), 633-638. https://doi.org/10.1016/j.stem.2011.05.001

Reprogramming of mouse and human cells to pluripotency using mature microRNAs. / Miyoshi, Norikatsu; Ishii, Hideshi; Nagano, Hiroaki; Haraguchi, Naotsugu; Dewi, Dyah Laksmi; Kano, Yoshihiro; Nishikawa, Shinpei; Tanemura, Masahiro; Mimori, Koshi; Tanaka, Fumiaki; Saito, Toshiyuki; Nishimura, Junichi; Takemasa, Ichiro; Mizushima, Tsunekazu; Ikeda, Masataka; Yamamoto, Hirofumi; Sekimoto, Mitsugu; Doki, Yuichiro; Mori, Masaki.

In: Cell stem cell, Vol. 8, No. 6, 03.06.2011, p. 633-638.

Research output: Contribution to journalArticle

Miyoshi, N, Ishii, H, Nagano, H, Haraguchi, N, Dewi, DL, Kano, Y, Nishikawa, S, Tanemura, M, Mimori, K, Tanaka, F, Saito, T, Nishimura, J, Takemasa, I, Mizushima, T, Ikeda, M, Yamamoto, H, Sekimoto, M, Doki, Y & Mori, M 2011, 'Reprogramming of mouse and human cells to pluripotency using mature microRNAs', Cell stem cell, vol. 8, no. 6, pp. 633-638. https://doi.org/10.1016/j.stem.2011.05.001
Miyoshi N, Ishii H, Nagano H, Haraguchi N, Dewi DL, Kano Y et al. Reprogramming of mouse and human cells to pluripotency using mature microRNAs. Cell stem cell. 2011 Jun 3;8(6):633-638. https://doi.org/10.1016/j.stem.2011.05.001
Miyoshi, Norikatsu ; Ishii, Hideshi ; Nagano, Hiroaki ; Haraguchi, Naotsugu ; Dewi, Dyah Laksmi ; Kano, Yoshihiro ; Nishikawa, Shinpei ; Tanemura, Masahiro ; Mimori, Koshi ; Tanaka, Fumiaki ; Saito, Toshiyuki ; Nishimura, Junichi ; Takemasa, Ichiro ; Mizushima, Tsunekazu ; Ikeda, Masataka ; Yamamoto, Hirofumi ; Sekimoto, Mitsugu ; Doki, Yuichiro ; Mori, Masaki. / Reprogramming of mouse and human cells to pluripotency using mature microRNAs. In: Cell stem cell. 2011 ; Vol. 8, No. 6. pp. 633-638.
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