TY - JOUR
T1 - Reprogramming of the histone H3.3 landscape in the early mouse embryo
AU - Ishiuchi, Takashi
AU - Abe, Shusaku
AU - Inoue, Kimiko
AU - Yeung, Wan Kin Au
AU - Miki, Yuka
AU - Ogura, Atsuo
AU - Sasaki, Hiroyuki
N1 - Funding Information:
We thank H. Sugishita and A. Inoue (RIKEN) for sharing recombinant pAG-MN protein and a CUT&RUN protocol, T. Wakayama (Yamanashi University) for sharing B6;129 F1 ESCs and K. Hayashi (Kyushu University) for sharing a confocal microscope. We also thank the members of our laboratory and common research facilities of the Medical Institute of Bioregulation, Kyushu University, for technical assistance. This work was supported by grants from a MEXT Grant-in-Aid for Scientific Research on Innovative Areas (JP19H05756 and JP19H05758; T.I. and A.O.), the Kato Memorial Bioscience Foundation (T.I.), a JSPS Grant-in-Aid for Scientific Research (B) (JP16H04687; K.I.) and a JSPS Grant-in-Aid for Specially Promoted Research (JP18H05214; H.S.)
PY - 2021/1
Y1 - 2021/1
N2 - Epigenetic reprogramming of the zygote involves dynamic incorporation of histone variant H3.3. However, the genome-wide distribution and dynamics of H3.3 during early development remain unknown. Here, we delineate the H3.3 landscapes in mouse oocytes and early embryos. We unexpectedly identify a non-canonical H3.3 pattern in mature oocytes and zygotes, in which local enrichment of H3.3 at active chromatin is suppressed and H3.3 is relatively evenly distributed across the genome. Interestingly, although the non-canonical H3.3 pattern forms gradually during oogenesis, it quickly switches to a canonical pattern at the two-cell stage in a transcription-independent and replication-dependent manner. We find that incorporation of H3.1/H3.2 mediated by chromatin assembly factor CAF-1 is a key process for the de novo establishment of the canonical pattern. Our data suggest that the presence of the non-canonical pattern and its timely transition toward a canonical pattern support the developmental program of early embryos.
AB - Epigenetic reprogramming of the zygote involves dynamic incorporation of histone variant H3.3. However, the genome-wide distribution and dynamics of H3.3 during early development remain unknown. Here, we delineate the H3.3 landscapes in mouse oocytes and early embryos. We unexpectedly identify a non-canonical H3.3 pattern in mature oocytes and zygotes, in which local enrichment of H3.3 at active chromatin is suppressed and H3.3 is relatively evenly distributed across the genome. Interestingly, although the non-canonical H3.3 pattern forms gradually during oogenesis, it quickly switches to a canonical pattern at the two-cell stage in a transcription-independent and replication-dependent manner. We find that incorporation of H3.1/H3.2 mediated by chromatin assembly factor CAF-1 is a key process for the de novo establishment of the canonical pattern. Our data suggest that the presence of the non-canonical pattern and its timely transition toward a canonical pattern support the developmental program of early embryos.
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U2 - 10.1038/s41594-020-00521-1
DO - 10.1038/s41594-020-00521-1
M3 - Article
C2 - 33169018
AN - SCOPUS:85095720082
VL - 28
SP - 38
EP - 49
JO - Nature Structural Biology
JF - Nature Structural Biology
SN - 1545-9993
IS - 1
ER -