Requirement for C-terminal extension to the RNA binding domain for efficient RNA binding by ribosomal protein L2

Takeshi Hayashi, Maino Tahara, Kenta Iwasaki, Yoshiaki Kouzuma, Makoto Kimura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ribosomal protein L2 is a primary 23S rRNA binding protein in the large ribosomal subunit. We examined the contribution of the N- and C-terminal regions of Bacillus stearothermophilus L2 (BstL2) to the 23S rRNA binding activity. The mutant desN, in which the N-terminal 59 residues of BstL2 were deleted, bound to the 23S rRNA fragment to the same extent as wild type BstL2, but the mutation desC, in which the C-terminal 74 amino acid residues were deleted, abolished the binding activity. These observations indicated that the C-terminal region is involved in 23S rRNA binding. Subsequent deletion analysis of the C-terminal region found that the C-terminal 70 amino acids are required for efficient 23S rRNA binding by BstL2. Furthermore, the surface plasmon resonance analysis indicated that successive truncations of the C-terminal residues increased the dissociation rate constants, while they had little influence on association rate constants. The result indicated that reduced affinities of the C-terminal deletion mutants were due only to higher dissociation rate constants, suggesting that the C-terminal region primarily functions by stabilizing the protein L2-23S rRNA complex.

Original languageEnglish
Pages (from-to)682-684
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number3
DOIs
Publication statusPublished - Jan 1 2002

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Geobacillus stearothermophilus
RNA-Binding Proteins
Bacilli
RNA
Rate constants
Large Ribosome Subunits
Amino Acids
Surface Plasmon Resonance
Surface plasmon resonance
Carrier Proteins
Association reactions
Mutation
RNA-Binding Motifs
ribosomal protein L2
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Requirement for C-terminal extension to the RNA binding domain for efficient RNA binding by ribosomal protein L2. / Hayashi, Takeshi; Tahara, Maino; Iwasaki, Kenta; Kouzuma, Yoshiaki; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 3, 01.01.2002, p. 682-684.

Research output: Contribution to journalArticle

Hayashi, Takeshi ; Tahara, Maino ; Iwasaki, Kenta ; Kouzuma, Yoshiaki ; Kimura, Makoto. / Requirement for C-terminal extension to the RNA binding domain for efficient RNA binding by ribosomal protein L2. In: Bioscience, Biotechnology and Biochemistry. 2002 ; Vol. 66, No. 3. pp. 682-684.
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