Abstract
Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.
| Original language | English |
|---|---|
| Pages (from-to) | 457-461 |
| Number of pages | 5 |
| Journal | Animal Science Journal |
| Volume | 87 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1 2016 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
Cite this
Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. / Nagatomo, Hiroaki; Kohri, Nanami; Akizawa, Hiroki; Hoshino, Yumi; Yamauchi, Nobuhiko; Kono, Tomohiro; Takahashi, Masashi; Kawahara, Manabu.
In: Animal Science Journal, Vol. 87, No. 3, 01.03.2016, p. 457-461.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development
AU - Nagatomo, Hiroaki
AU - Kohri, Nanami
AU - Akizawa, Hiroki
AU - Hoshino, Yumi
AU - Yamauchi, Nobuhiko
AU - Kono, Tomohiro
AU - Takahashi, Masashi
AU - Kawahara, Manabu
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.
AB - Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.
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UR - http://www.scopus.com/inward/citedby.url?scp=84960497448&partnerID=8YFLogxK
U2 - 10.1111/asj.12538
DO - 10.1111/asj.12538
M3 - Article
C2 - 26690724
AN - SCOPUS:84960497448
VL - 87
SP - 457
EP - 461
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 3
ER -