Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Hiroaki Nagatomo, Nanami Kohri, Hiroki Akizawa, Yumi Hoshino, Nobuhiko Yamauchi, Tomohiro Kono, Masashi Takahashi, Manabu Kawahara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalAnimal Science Journal
Volume87
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

messenger RNA
Spermatozoa
spermatozoa
Messenger RNA
cattle
Blastocyst
blastocyst
Proteins
proteins
Embryonic Development
embryogenesis
Gene Knockdown Techniques
DNA replication
DNA Replication
histones
Histones
cell cycle
cell proliferation
Cell Cycle
embryo (animal)

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. / Nagatomo, Hiroaki; Kohri, Nanami; Akizawa, Hiroki; Hoshino, Yumi; Yamauchi, Nobuhiko; Kono, Tomohiro; Takahashi, Masashi; Kawahara, Manabu.

In: Animal Science Journal, Vol. 87, No. 3, 01.03.2016, p. 457-461.

Research output: Contribution to journalArticle

Nagatomo, H, Kohri, N, Akizawa, H, Hoshino, Y, Yamauchi, N, Kono, T, Takahashi, M & Kawahara, M 2016, 'Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development', Animal Science Journal, vol. 87, no. 3, pp. 457-461. https://doi.org/10.1111/asj.12538
Nagatomo, Hiroaki ; Kohri, Nanami ; Akizawa, Hiroki ; Hoshino, Yumi ; Yamauchi, Nobuhiko ; Kono, Tomohiro ; Takahashi, Masashi ; Kawahara, Manabu. / Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. In: Animal Science Journal. 2016 ; Vol. 87, No. 3. pp. 457-461.
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