TY - JOUR
T1 - Rescue system for measles virus from cloned cDNA driven by vaccinia virus Lister vaccine strain
AU - Nakatsu, Yuichiro
AU - Takeda, Makoto
AU - Kidokoro, Minoru
AU - Kohara, Michinori
AU - Yanagi, Yusuke
N1 - Funding Information:
We thank Drs. B. Moss, K. Komase, and M. Nagano for providing the recombinant vaccinia viruses vTF7-3 and MVA-T7, the minigenome plasmid, and pBS-GFP, respectively. We also thank Dr. H. Minagawa for helpful discussions, and Dr. A. P. Schmitt for critical reading and helpful comments. This work was supported by grants from the Ministry of Education, Science, and Culture and the Ministry of Health, Labor, and Welfare of Japan, the Japan Society for the Promotion of Science.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2006/10
Y1 - 2006/10
N2 - A rescue system for measles virus from cloned cDNA was established using CHO/hSLAM cells (Chinese hamster ovary cells expressing a measles virus receptor, signaling lymphocyte activation molecule), LO-T7-1 virus (the Lister vaccine strain of vaccinia virus expressing the T7 RNA polymerase under the control of the early/late p7.5 promoter), and caspase inhibitor. LO-T7-1 drove efficiently the T7 promoter in CHO/hSLAM cells. Rescue efficiency with LO-T7-1 was not as high as that with the vTF7-3 strain based on a neurovirulent vaccinia virus, but was increased by using a caspase inhibitor to block apoptosis of CHO/hSLAM cells induced by LO-T7-1. These modifications resulted in a measles virus rescue efficiency that was even higher than that of previous systems. This safer and more efficient system will facilitate further the genetic manipulation of measles virus in basic research and virus vector development.
AB - A rescue system for measles virus from cloned cDNA was established using CHO/hSLAM cells (Chinese hamster ovary cells expressing a measles virus receptor, signaling lymphocyte activation molecule), LO-T7-1 virus (the Lister vaccine strain of vaccinia virus expressing the T7 RNA polymerase under the control of the early/late p7.5 promoter), and caspase inhibitor. LO-T7-1 drove efficiently the T7 promoter in CHO/hSLAM cells. Rescue efficiency with LO-T7-1 was not as high as that with the vTF7-3 strain based on a neurovirulent vaccinia virus, but was increased by using a caspase inhibitor to block apoptosis of CHO/hSLAM cells induced by LO-T7-1. These modifications resulted in a measles virus rescue efficiency that was even higher than that of previous systems. This safer and more efficient system will facilitate further the genetic manipulation of measles virus in basic research and virus vector development.
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U2 - 10.1016/j.jviromet.2006.05.029
DO - 10.1016/j.jviromet.2006.05.029
M3 - Article
C2 - 16854475
AN - SCOPUS:33746870434
SN - 0166-0934
VL - 137
SP - 152
EP - 155
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -
