Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody

Yoshikazu Fujimoto, Kinuyo Ozaki, Masahiro Maeda, Ken ichi Nishijima, Hiroki Takakuwa, Koichi Otsuki, Hiroshi Kida, Etsuro Ono

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The polymerase basic 2 (PB2) protein is one of four proteins that make up the influenza A virus replication complex, which is responsible for viral gene transcription and replication. To assess the antiviral potential of an anti-PB2 monoclonal antibody that inhibits RNA transcription of influenza A viruses, Mardin-Darby canine kidney (MDCK) cells were transformed with two transgenes that encode the light and heavy chains of the monoclonal antibody. The transformed cell lines expressing this monoclonal antibody displayed resistance to several subtypes of influenza A virus infection. In the transformed cell lines infected with influenza A virus, the level of viral RNA transcription was decreased and the effective nuclear transportation of the PB2 protein was also inhibited. These results demonstrate that the anti-PB2 intrabody is potentially able to interfere with the effective nuclear transportation of PB2 protein, resulting in the observed resistance to influenza A virus infection in vitro.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalVeterinary Journal
Volume198
Issue number2
DOIs
Publication statusPublished - Nov 2013

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody'. Together they form a unique fingerprint.

  • Cite this