Resolution and characterization of tryptophyl fluorescence of hen egg-white lysozyme by quenching- and time-resolved spectroscopy

Etsuko Nishimoto, Shoji Yamashita, Nobuyuki Yamasaki, Taiji Imoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The fluorescence spectral distributions of four tryptophan residues of hen egg-white lysozyme were analyzed using time-resolved and quenching-resolved fluorescence spectroscopy. Trp62 and Trp108 gave the fluorescence maxima at 352 nm and 342 nm, respectively. The fluorescence of Trp28 and Trp111 occurred only at 300-360 nm and they were observed as an unresolved emission band with a maximum and shoulder at 320 nm and 330 nm. The fluorescence quenching and decay parameters of each tryptophan residue reconfirmed that Trp62 was fully exposed to the solvent but Trp108 was sealed in the cage of the peptide chains and furthermore showed that Trp28 and Trp111 are under the influence of the larger fluctuational motion at the hydrophobic matrix box. The fluorescence responses of each tryptophan residue to the lysozyme-ligand interaction suggested that the internal fluctuation was reduced by the binding of ligand to give a distorted conformation to the hydrophobic matrix box region.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number2
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Egg White
Muramidase
Quenching
Spectrum Analysis
Fluorescence
Spectroscopy
Tryptophan
Ligands
Fluorescence Spectrometry
Fluorescence spectroscopy
Conformations
hen egg lysozyme
Peptides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Resolution and characterization of tryptophyl fluorescence of hen egg-white lysozyme by quenching- and time-resolved spectroscopy. / Nishimoto, Etsuko; Yamashita, Shoji; Yamasaki, Nobuyuki; Imoto, Taiji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 2, 01.01.1999, p. 329-336.

Research output: Contribution to journalArticle

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