Response of cells on surface-induced nanopatterns: Fibroblasts and mesenchymal progenitor cells

Hwei Ling Khor, Yujun Kuan, Hildegard Kukula, Kaoru Tamada, Wolfgang Knoll, Martin Moeller, Dietmar W. Hutmacher

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Ultrathin films of a poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer (PS-b-P2VP) and poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer (PS-b-P4VP) were used to form surface-induced nanopattern (SINPAT) on mica. Surface interaction controlled microphase separation led to the formation of chemically heterogeneous surface nanopatterns on dry ultrathin films. Two distinct nanopatterned surfaces, namely, wormlike and dotlike patterns, were used to investigate the influence of topography in the nanometer range on cell adhesion, proliferation, and migration. Atomic force microscopy was used to confirm that SINPAT was stable under cell culture conditions. Fibroblasts and mesenchymal progenitor cells were cultured on the nanopatterned surfaces. Phase contrast and confocal laser microscopy showed that fibroblasts and mesenchymal progenitor cells preferred the densely spaced wormlike patterns. Atomic force microscopy showed that the cells remodelled the extracellular matrix differently as they migrate over the two distinctly different nanopatterns.

Original languageEnglish
Pages (from-to)1530-1540
Number of pages11
JournalBiomacromolecules
Volume8
Issue number5
DOIs
Publication statusPublished - May 1 2007
Externally publishedYes

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Atomic Force Microscopy
Fibroblasts
Mesenchymal Stromal Cells
Confocal Microscopy
Cell Adhesion
Cell Movement
Extracellular Matrix
Cell Culture Techniques
Ultrathin films
Cell Proliferation
Block copolymers
Styrene
Atomic force microscopy
Microphase separation
Cell adhesion
Mica
Cell culture
Topography
Microscopic examination
Lasers

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Khor, H. L., Kuan, Y., Kukula, H., Tamada, K., Knoll, W., Moeller, M., & Hutmacher, D. W. (2007). Response of cells on surface-induced nanopatterns: Fibroblasts and mesenchymal progenitor cells. Biomacromolecules, 8(5), 1530-1540. https://doi.org/10.1021/bm0611533

Response of cells on surface-induced nanopatterns : Fibroblasts and mesenchymal progenitor cells. / Khor, Hwei Ling; Kuan, Yujun; Kukula, Hildegard; Tamada, Kaoru; Knoll, Wolfgang; Moeller, Martin; Hutmacher, Dietmar W.

In: Biomacromolecules, Vol. 8, No. 5, 01.05.2007, p. 1530-1540.

Research output: Contribution to journalArticle

Khor, HL, Kuan, Y, Kukula, H, Tamada, K, Knoll, W, Moeller, M & Hutmacher, DW 2007, 'Response of cells on surface-induced nanopatterns: Fibroblasts and mesenchymal progenitor cells', Biomacromolecules, vol. 8, no. 5, pp. 1530-1540. https://doi.org/10.1021/bm0611533
Khor, Hwei Ling ; Kuan, Yujun ; Kukula, Hildegard ; Tamada, Kaoru ; Knoll, Wolfgang ; Moeller, Martin ; Hutmacher, Dietmar W. / Response of cells on surface-induced nanopatterns : Fibroblasts and mesenchymal progenitor cells. In: Biomacromolecules. 2007 ; Vol. 8, No. 5. pp. 1530-1540.
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