Response regulator YycF essential for bacterial growth

X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif

Toshihide Okajima, Akihiro Doi, Ario Okada, Yasuhiro Gotoh, Katsuyuki Tanizawa, Ryutaro Utsumi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.

Original languageEnglish
Pages (from-to)3434-3438
Number of pages5
JournalFEBS Letters
Volume582
Issue number23-24
DOIs
Publication statusPublished - Oct 15 2008
Externally publishedYes

Fingerprint

Helix-Turn-Helix Motifs
Signal transduction
Nucleotide Motifs
Base Composition
Gram-Positive Bacteria
Bacilli
Bacillus subtilis
Escherichia coli
Alkaline Phosphatase
Signal Transduction
Bacteria
Phosphotransferases
Crystal structure
X-Rays
X rays
DNA
Sensors
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Response regulator YycF essential for bacterial growth : X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif. / Okajima, Toshihide; Doi, Akihiro; Okada, Ario; Gotoh, Yasuhiro; Tanizawa, Katsuyuki; Utsumi, Ryutaro.

In: FEBS Letters, Vol. 582, No. 23-24, 15.10.2008, p. 3434-3438.

Research output: Contribution to journalArticle

Okajima, Toshihide ; Doi, Akihiro ; Okada, Ario ; Gotoh, Yasuhiro ; Tanizawa, Katsuyuki ; Utsumi, Ryutaro. / Response regulator YycF essential for bacterial growth : X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif. In: FEBS Letters. 2008 ; Vol. 582, No. 23-24. pp. 3434-3438.
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