Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes

Quynh Vu, Reyuel Quintana, Daisuke Fujita, Carmencita C. Bernal, Hideshi Yasui, Celia D. Medina, Finbarr G. Horgan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The green leafhopper, Nephotettix virescens (Distant) (Hemiptera: Cicadellidae), occasionally damages rice in Asia either directly, by feeding on the host phloem, or indirectly by transmitting tungro virus. We assessed the nature of resistance against the leafhopper in monogenic and pyramided near-isogenic rice lines containing the resistance genes Grh2 and Grh4. Only the pyramided line was resistant to leafhopper damage. Leafhopper nymphs and adults had high mortality and low weight gain when feeding on the pyramided line and adults laid few eggs. In contrast, although there was some minor resistance in 45-day-old plants that possessed either Grh2 or Grh4 genes, the monogenic lines were generally as susceptible to the leafhopper as the recurrent parent line Taichung65 (T65). Resistance in the pyramided line was stable as the plant aged and under high nitrogen, and affected each of five Philippine leafhopper populations equally. Furthermore, in a selection study, leafhoppers failed to adapt fully to the pyramided resistant line: nymph and adult survival did improve during the first five generations of selection and attained similar levels as on T65, but egg-laying failed to improve over 10 generations. Our preliminary results suggested that resistance was associated with physiological costs to the plants in some experiments. The results of this study demonstrate the success of pyramiding resistance genes through marker-assisted breeding, to achieve a strong and potentially durable resistance. We discuss the utility of gene pyramiding and the development of near-isogenic lines for leafhopper management.

Original languageEnglish
Pages (from-to)179-190
Number of pages12
JournalEntomologia Experimentalis et Applicata
Volume150
Issue number2
DOIs
Publication statusPublished - Feb 1 2014

Fingerprint

Nephotettix virescens
Cicadellidae
rice
gene
genes
egg
damage
phloem
virus
nymphs
breeding
mortality
nitrogen
cost
isogenic lines
Philippines
experiment
Hemiptera
oviposition
weight gain

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes. / Vu, Quynh; Quintana, Reyuel; Fujita, Daisuke; Bernal, Carmencita C.; Yasui, Hideshi; Medina, Celia D.; Horgan, Finbarr G.

In: Entomologia Experimentalis et Applicata, Vol. 150, No. 2, 01.02.2014, p. 179-190.

Research output: Contribution to journalArticle

Vu, Quynh ; Quintana, Reyuel ; Fujita, Daisuke ; Bernal, Carmencita C. ; Yasui, Hideshi ; Medina, Celia D. ; Horgan, Finbarr G. / Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes. In: Entomologia Experimentalis et Applicata. 2014 ; Vol. 150, No. 2. pp. 179-190.
@article{d7080a99562a407c91aba844cdbe03ea,
title = "Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes",
abstract = "The green leafhopper, Nephotettix virescens (Distant) (Hemiptera: Cicadellidae), occasionally damages rice in Asia either directly, by feeding on the host phloem, or indirectly by transmitting tungro virus. We assessed the nature of resistance against the leafhopper in monogenic and pyramided near-isogenic rice lines containing the resistance genes Grh2 and Grh4. Only the pyramided line was resistant to leafhopper damage. Leafhopper nymphs and adults had high mortality and low weight gain when feeding on the pyramided line and adults laid few eggs. In contrast, although there was some minor resistance in 45-day-old plants that possessed either Grh2 or Grh4 genes, the monogenic lines were generally as susceptible to the leafhopper as the recurrent parent line Taichung65 (T65). Resistance in the pyramided line was stable as the plant aged and under high nitrogen, and affected each of five Philippine leafhopper populations equally. Furthermore, in a selection study, leafhoppers failed to adapt fully to the pyramided resistant line: nymph and adult survival did improve during the first five generations of selection and attained similar levels as on T65, but egg-laying failed to improve over 10 generations. Our preliminary results suggested that resistance was associated with physiological costs to the plants in some experiments. The results of this study demonstrate the success of pyramiding resistance genes through marker-assisted breeding, to achieve a strong and potentially durable resistance. We discuss the utility of gene pyramiding and the development of near-isogenic lines for leafhopper management.",
author = "Quynh Vu and Reyuel Quintana and Daisuke Fujita and Bernal, {Carmencita C.} and Hideshi Yasui and Medina, {Celia D.} and Horgan, {Finbarr G.}",
year = "2014",
month = "2",
day = "1",
doi = "10.1111/eea.12149",
language = "English",
volume = "150",
pages = "179--190",
journal = "Entomologia Experimentalis et Applicata",
issn = "0013-8703",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes

AU - Vu, Quynh

AU - Quintana, Reyuel

AU - Fujita, Daisuke

AU - Bernal, Carmencita C.

AU - Yasui, Hideshi

AU - Medina, Celia D.

AU - Horgan, Finbarr G.

PY - 2014/2/1

Y1 - 2014/2/1

N2 - The green leafhopper, Nephotettix virescens (Distant) (Hemiptera: Cicadellidae), occasionally damages rice in Asia either directly, by feeding on the host phloem, or indirectly by transmitting tungro virus. We assessed the nature of resistance against the leafhopper in monogenic and pyramided near-isogenic rice lines containing the resistance genes Grh2 and Grh4. Only the pyramided line was resistant to leafhopper damage. Leafhopper nymphs and adults had high mortality and low weight gain when feeding on the pyramided line and adults laid few eggs. In contrast, although there was some minor resistance in 45-day-old plants that possessed either Grh2 or Grh4 genes, the monogenic lines were generally as susceptible to the leafhopper as the recurrent parent line Taichung65 (T65). Resistance in the pyramided line was stable as the plant aged and under high nitrogen, and affected each of five Philippine leafhopper populations equally. Furthermore, in a selection study, leafhoppers failed to adapt fully to the pyramided resistant line: nymph and adult survival did improve during the first five generations of selection and attained similar levels as on T65, but egg-laying failed to improve over 10 generations. Our preliminary results suggested that resistance was associated with physiological costs to the plants in some experiments. The results of this study demonstrate the success of pyramiding resistance genes through marker-assisted breeding, to achieve a strong and potentially durable resistance. We discuss the utility of gene pyramiding and the development of near-isogenic lines for leafhopper management.

AB - The green leafhopper, Nephotettix virescens (Distant) (Hemiptera: Cicadellidae), occasionally damages rice in Asia either directly, by feeding on the host phloem, or indirectly by transmitting tungro virus. We assessed the nature of resistance against the leafhopper in monogenic and pyramided near-isogenic rice lines containing the resistance genes Grh2 and Grh4. Only the pyramided line was resistant to leafhopper damage. Leafhopper nymphs and adults had high mortality and low weight gain when feeding on the pyramided line and adults laid few eggs. In contrast, although there was some minor resistance in 45-day-old plants that possessed either Grh2 or Grh4 genes, the monogenic lines were generally as susceptible to the leafhopper as the recurrent parent line Taichung65 (T65). Resistance in the pyramided line was stable as the plant aged and under high nitrogen, and affected each of five Philippine leafhopper populations equally. Furthermore, in a selection study, leafhoppers failed to adapt fully to the pyramided resistant line: nymph and adult survival did improve during the first five generations of selection and attained similar levels as on T65, but egg-laying failed to improve over 10 generations. Our preliminary results suggested that resistance was associated with physiological costs to the plants in some experiments. The results of this study demonstrate the success of pyramiding resistance genes through marker-assisted breeding, to achieve a strong and potentially durable resistance. We discuss the utility of gene pyramiding and the development of near-isogenic lines for leafhopper management.

UR - http://www.scopus.com/inward/record.url?scp=84892528774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892528774&partnerID=8YFLogxK

U2 - 10.1111/eea.12149

DO - 10.1111/eea.12149

M3 - Article

AN - SCOPUS:84892528774

VL - 150

SP - 179

EP - 190

JO - Entomologia Experimentalis et Applicata

JF - Entomologia Experimentalis et Applicata

SN - 0013-8703

IS - 2

ER -