Responses to apical and basolateral application of glutamate in mouse fungiform taste cells with action potentials.

Mayu Niki, Shingo Takai, Yoko Kusuhara, Yuzo Ninomiya, Ryusuke Yoshida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In taste bud cells, glutamate may elicit two types of responses, as an umami tastant and as a neurotransmitter. Glutamate applied to apical membrane of taste cells would elicit taste responses whereas glutamate applied to basolateral membrane may act as a neurotransmitter. Using restricted stimulation to apical or basolateral membrane of taste cells, we examined responses of taste cells to glutamate stimulation, separately. Apical application of monosodium glutamate (MSG, 0.3 M) increased firing frequency in some of mouse fungiform taste cells that evoked action potentials. These cells were tested with other basic taste compounds, NaCl (salty), saccharin (sweet), HCl (sour), and quinine (bitter). MSG-sensitive taste cells could be classified into sweet-best (S-type), MSG-best (M-type), and NaCl or other electrolytes-best (N- or E/H-type) cells. Furthermore, S- and M-type could be classified into two sub-types according to the synergistic effect between MSG and inosine-5'-monophosphate (S1, M1 with synergism; S2, M2 without synergism). Basolateral application of glutamate (100 μM) had almost no effect on the mean spontaneous firing rates in taste cells. However, about 10% of taste cells tested showed transient increases in spontaneous firing rates (>mean + 2 standard deviation) after basolateral application of glutamate. These results suggest the existence of multiple types of umami-sensitive taste cells and the existence of glutamate receptor(s) on the basolateral membrane of a subset of taste cells.

Original languageEnglish
Pages (from-to)1033-1040
Number of pages8
JournalCellular and molecular neurobiology
Volume31
Issue number7
DOIs
Publication statusPublished - Jan 1 2011

Fingerprint

Action Potentials
Glutamic Acid
Sodium Glutamate
Neurotransmitter Agents
Cell Membrane
Taste Buds
Saccharin
Inosine Monophosphate
Quinine
Membranes
Glutamate Receptors
Evoked Potentials
Electrolytes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Responses to apical and basolateral application of glutamate in mouse fungiform taste cells with action potentials. / Niki, Mayu; Takai, Shingo; Kusuhara, Yoko; Ninomiya, Yuzo; Yoshida, Ryusuke.

In: Cellular and molecular neurobiology, Vol. 31, No. 7, 01.01.2011, p. 1033-1040.

Research output: Contribution to journalArticle

Niki, Mayu ; Takai, Shingo ; Kusuhara, Yoko ; Ninomiya, Yuzo ; Yoshida, Ryusuke. / Responses to apical and basolateral application of glutamate in mouse fungiform taste cells with action potentials. In: Cellular and molecular neurobiology. 2011 ; Vol. 31, No. 7. pp. 1033-1040.
@article{9b08174c54144392a45694e5bbfd2f80,
title = "Responses to apical and basolateral application of glutamate in mouse fungiform taste cells with action potentials.",
abstract = "In taste bud cells, glutamate may elicit two types of responses, as an umami tastant and as a neurotransmitter. Glutamate applied to apical membrane of taste cells would elicit taste responses whereas glutamate applied to basolateral membrane may act as a neurotransmitter. Using restricted stimulation to apical or basolateral membrane of taste cells, we examined responses of taste cells to glutamate stimulation, separately. Apical application of monosodium glutamate (MSG, 0.3 M) increased firing frequency in some of mouse fungiform taste cells that evoked action potentials. These cells were tested with other basic taste compounds, NaCl (salty), saccharin (sweet), HCl (sour), and quinine (bitter). MSG-sensitive taste cells could be classified into sweet-best (S-type), MSG-best (M-type), and NaCl or other electrolytes-best (N- or E/H-type) cells. Furthermore, S- and M-type could be classified into two sub-types according to the synergistic effect between MSG and inosine-5'-monophosphate (S1, M1 with synergism; S2, M2 without synergism). Basolateral application of glutamate (100 μM) had almost no effect on the mean spontaneous firing rates in taste cells. However, about 10{\%} of taste cells tested showed transient increases in spontaneous firing rates (>mean + 2 standard deviation) after basolateral application of glutamate. These results suggest the existence of multiple types of umami-sensitive taste cells and the existence of glutamate receptor(s) on the basolateral membrane of a subset of taste cells.",
author = "Mayu Niki and Shingo Takai and Yoko Kusuhara and Yuzo Ninomiya and Ryusuke Yoshida",
year = "2011",
month = "1",
day = "1",
doi = "10.1007/s10571-011-9702-5",
language = "English",
volume = "31",
pages = "1033--1040",
journal = "Cellular and Molecular Neurobiology",
issn = "0272-4340",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Responses to apical and basolateral application of glutamate in mouse fungiform taste cells with action potentials.

AU - Niki, Mayu

AU - Takai, Shingo

AU - Kusuhara, Yoko

AU - Ninomiya, Yuzo

AU - Yoshida, Ryusuke

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In taste bud cells, glutamate may elicit two types of responses, as an umami tastant and as a neurotransmitter. Glutamate applied to apical membrane of taste cells would elicit taste responses whereas glutamate applied to basolateral membrane may act as a neurotransmitter. Using restricted stimulation to apical or basolateral membrane of taste cells, we examined responses of taste cells to glutamate stimulation, separately. Apical application of monosodium glutamate (MSG, 0.3 M) increased firing frequency in some of mouse fungiform taste cells that evoked action potentials. These cells were tested with other basic taste compounds, NaCl (salty), saccharin (sweet), HCl (sour), and quinine (bitter). MSG-sensitive taste cells could be classified into sweet-best (S-type), MSG-best (M-type), and NaCl or other electrolytes-best (N- or E/H-type) cells. Furthermore, S- and M-type could be classified into two sub-types according to the synergistic effect between MSG and inosine-5'-monophosphate (S1, M1 with synergism; S2, M2 without synergism). Basolateral application of glutamate (100 μM) had almost no effect on the mean spontaneous firing rates in taste cells. However, about 10% of taste cells tested showed transient increases in spontaneous firing rates (>mean + 2 standard deviation) after basolateral application of glutamate. These results suggest the existence of multiple types of umami-sensitive taste cells and the existence of glutamate receptor(s) on the basolateral membrane of a subset of taste cells.

AB - In taste bud cells, glutamate may elicit two types of responses, as an umami tastant and as a neurotransmitter. Glutamate applied to apical membrane of taste cells would elicit taste responses whereas glutamate applied to basolateral membrane may act as a neurotransmitter. Using restricted stimulation to apical or basolateral membrane of taste cells, we examined responses of taste cells to glutamate stimulation, separately. Apical application of monosodium glutamate (MSG, 0.3 M) increased firing frequency in some of mouse fungiform taste cells that evoked action potentials. These cells were tested with other basic taste compounds, NaCl (salty), saccharin (sweet), HCl (sour), and quinine (bitter). MSG-sensitive taste cells could be classified into sweet-best (S-type), MSG-best (M-type), and NaCl or other electrolytes-best (N- or E/H-type) cells. Furthermore, S- and M-type could be classified into two sub-types according to the synergistic effect between MSG and inosine-5'-monophosphate (S1, M1 with synergism; S2, M2 without synergism). Basolateral application of glutamate (100 μM) had almost no effect on the mean spontaneous firing rates in taste cells. However, about 10% of taste cells tested showed transient increases in spontaneous firing rates (>mean + 2 standard deviation) after basolateral application of glutamate. These results suggest the existence of multiple types of umami-sensitive taste cells and the existence of glutamate receptor(s) on the basolateral membrane of a subset of taste cells.

UR - http://www.scopus.com/inward/record.url?scp=85027949062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027949062&partnerID=8YFLogxK

U2 - 10.1007/s10571-011-9702-5

DO - 10.1007/s10571-011-9702-5

M3 - Article

C2 - 21573975

AN - SCOPUS:85027949062

VL - 31

SP - 1033

EP - 1040

JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

SN - 0272-4340

IS - 7

ER -