Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes

Yoichi Shinkai, Shigeo Koyasu, Kei Ichi Nakayama, Kenneth M. Murphy, Dennis Y. Loh, Ellis L. Reinherz, Frederick W. Alt

Research output: Contribution to journalArticlepeer-review

357 Citations (Scopus)

Abstract

Introduction of TCRα transgene, TCRβ transgene, or both into RAG-2-/- mice differentially rescues T cell development. RAG-2 -/- mice have small numbers of TCR-CD4-CD8 - (double negative, DN) thymocytes that express CD3γδ ∈ and ζ proteins intracellularly. Introduction of a TCRβ transgene, but not a TCRα transgene, into the RAG-2-/- back-ground restored normal numbers of thymocytes. These cells were CD4 +CD8+ (double positive, DP) and expressed small amounts of surface TCRβ chain dimers in association with CD3γδ∈ but not ζ. RAG-2-/- mice that expressed α and β TCR transgenes developed both DP and single positive thymocytes. Thus, the TCRβ subunit, possibly in association with a novel CD3 complex, participates in the DN to the DP transition.

Original languageEnglish
Pages (from-to)822-825
Number of pages4
JournalScience
Volume259
Issue number5096
DOIs
Publication statusPublished - 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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