Restoration of the optimal redox state for the photosynthetic electron transfer system by auxiliary oxidants in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114

Ken ichiro Takamiya, Hiroyuki Arata, Yuzo Shioi, Michio Doi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114, photosynthetic electron transfer (photooxidation of cytochromes) and light-driven proton release did not occur under anaerobic conditions. 'Auxiliary oxidants', such as nitrate, nitrite, trimethylamine N-oxide (TMAO) and chlorate, restored the optimal redox state for the photosynthetic electron transfer system, resulting in the photooxidation of c-type cytochrome and the release of protons from intact cells on illumination. These auxiliary oxidants oxidized c-type cytochrome(s) under anaerobic conditions in the dark, suggesting the presence of reductases for these auxiliary oxidants. The redox level of the electron transfer chain shifted towards oxidation in the presence of the auxiliary oxidants and was poised to allow photosynthetic electron transfer. KCN inhibited the photooxidation of cytochromes and the light-driven proton release in the presence of these auxiliary oxidants, except for TMAO. KCN also inhibited the auxiliary oxidant-induced oxidation of cytochromes. This suggests that KCN inhibits the reductases for these oxidants. Antimycin and myxothiazol enhanced the auxiliary oxidant-induced oxidation of cytochrome(s), suggesting the involvement of the cytochrome b-c1 complex in nitrate, chlorate and nitrite reduction.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
JournalBBA - Bioenergetics
Volume935
Issue number1
DOIs
Publication statusPublished - Aug 17 1988

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Sphingomonadaceae
Aerobic Bacteria
Oxidants
Restoration
Oxidation-Reduction
Bacteria
Electrons
Cytochromes
Photooxidation
Chlorates
Cytochrome c Group
Protons
Nitrites
Nitrates
Oxidation
Oxidoreductases
Cytochromes c1
Light
Cytochromes b
Lighting

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Restoration of the optimal redox state for the photosynthetic electron transfer system by auxiliary oxidants in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. / Takamiya, Ken ichiro; Arata, Hiroyuki; Shioi, Yuzo; Doi, Michio.

In: BBA - Bioenergetics, Vol. 935, No. 1, 17.08.1988, p. 26-33.

Research output: Contribution to journalArticle

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