Restorative effects of human neural stem cell grafts on the primate spinal cord

Ephron S. Rosenzweig, John H. Brock, Paul Lu, Hiromi Kumamaru, Ernesto A. Salegio, Ken Kadoya, Janet L. Weber, Justine J. Liang, Rod Moseanko, Stephanie Hawbecker, J. Russell Huie, Leif A. Havton, Yvette S. Nout-Lomas, Adam R. Ferguson, Michael S. Beattie, Jacqueline C. Bresnahan, Mark H. Tuszynski

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We grafted human spinal cord-derived neural progenitor cells (NPCs) into sites of cervical spinal cord injury in rhesus monkeys (Macaca mulatta). Under three-drug immunosuppression, grafts survived at least 9 months postinjury and expressed both neuronal and glial markers. Monkey axons regenerated into grafts and formed synapses. Hundreds of thousands of human axons extended out from grafts through monkey white matter and synapsed in distal gray matter. Grafts gradually matured over 9 months and improved forelimb function beginning several months after grafting. These findings in a 'preclinical trial' support translation of NPC graft therapy to humans with the objective of reconstituting both a neuronal and glial milieu in the site of spinal cord injury.

Original languageEnglish
Pages (from-to)484-490
Number of pages7
JournalNature medicine
Volume24
Issue number4
DOIs
Publication statusPublished - May 1 2018

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Neural Stem Cells
Stem cells
Grafts
Primates
Spinal Cord
Transplants
Macaca mulatta
Spinal Cord Injuries
Neuroglia
Haplorhini
Axons
Stem Cells
Forelimb
Cell- and Tissue-Based Therapy
Synapses
Immunosuppression
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rosenzweig, E. S., Brock, J. H., Lu, P., Kumamaru, H., Salegio, E. A., Kadoya, K., ... Tuszynski, M. H. (2018). Restorative effects of human neural stem cell grafts on the primate spinal cord. Nature medicine, 24(4), 484-490. https://doi.org/10.1038/nm.4502

Restorative effects of human neural stem cell grafts on the primate spinal cord. / Rosenzweig, Ephron S.; Brock, John H.; Lu, Paul; Kumamaru, Hiromi; Salegio, Ernesto A.; Kadoya, Ken; Weber, Janet L.; Liang, Justine J.; Moseanko, Rod; Hawbecker, Stephanie; Huie, J. Russell; Havton, Leif A.; Nout-Lomas, Yvette S.; Ferguson, Adam R.; Beattie, Michael S.; Bresnahan, Jacqueline C.; Tuszynski, Mark H.

In: Nature medicine, Vol. 24, No. 4, 01.05.2018, p. 484-490.

Research output: Contribution to journalArticle

Rosenzweig, ES, Brock, JH, Lu, P, Kumamaru, H, Salegio, EA, Kadoya, K, Weber, JL, Liang, JJ, Moseanko, R, Hawbecker, S, Huie, JR, Havton, LA, Nout-Lomas, YS, Ferguson, AR, Beattie, MS, Bresnahan, JC & Tuszynski, MH 2018, 'Restorative effects of human neural stem cell grafts on the primate spinal cord', Nature medicine, vol. 24, no. 4, pp. 484-490. https://doi.org/10.1038/nm.4502
Rosenzweig, Ephron S. ; Brock, John H. ; Lu, Paul ; Kumamaru, Hiromi ; Salegio, Ernesto A. ; Kadoya, Ken ; Weber, Janet L. ; Liang, Justine J. ; Moseanko, Rod ; Hawbecker, Stephanie ; Huie, J. Russell ; Havton, Leif A. ; Nout-Lomas, Yvette S. ; Ferguson, Adam R. ; Beattie, Michael S. ; Bresnahan, Jacqueline C. ; Tuszynski, Mark H. / Restorative effects of human neural stem cell grafts on the primate spinal cord. In: Nature medicine. 2018 ; Vol. 24, No. 4. pp. 484-490.
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