Restricted diversity of the variable region nucleotide sequences of the heavy and light chains of a human rheumatoid factor

Ichiko Ezaki, Masao Shingu, Masashi Nobunaga, Hidetoshi Kanda, Takeshi Watanabe, Kouko Sakai, Nobunori Fukui

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

The complete nucleotide sequences of the variable region genes of the heavy and light polypeptide chains of a human monoclonal rheumatoid factor (RF) produced from a human–mouse heterohybridoma were determined. The antibody, designated YES8c, contained V κIII, Jκ2, VH1, JH4, and a D gene segment of 9 amino acids. The nucleotide sequences and the deduced amino acid sequences of the light chain variable region were remarkably homologous (97–98%) to previously described RF of the Wa idiotypic family (PAY, GLO, CUR, FLO, and GAR) and to that of a VκIII germline gene (Humkv325). The YES8c heavy chain variable region gene was most closely related to the VH1 gene of the restricted human fetal repertoire, designated 51p1, and also to 3 rearranged VH1 genes that were recently isolated from patients with chronic lymphocytic leukemia. These results suggest that variable region genes of RFs are highly conserved and that YES8c VH, as well as Vκ, may be identical to heavy and light chains expressed during early B cell development.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalArthritis & Rheumatism
Volume34
Issue number3
DOIs
Publication statusPublished - Mar 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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