Restricted occurrence of an unusual ganglioside GD1α in rat brain and its possible involvement in dendritic growth of cerebellar Purkinje neurons

Shigeki Furuya, T. Hashikawa, Y. Hirabayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The spatial pattern of expression of a minor disialosyl ganglioside GD1α in the rat brain was investigated immunohistochemically using a specific murine monoclonal antibody KA-17. The antibody shows noticeable immunoreactivity in the proximal dendrites and neuronal cell bodies of restricted populations of neurons including cerebral pyramidal neurons and cerebellar Purkinje neurons. Immunocytochemical analysis revealed that Purkinje neurons maintained in a dissociate culture condition also express GD1α in the dendrites and cell bodies. We have examined the functional involvement of this ganglioside in the growth of brain neurons using KA-17 antibody. Addition of the antibody to cerebellar primary cultures caused perturbation of the dendritic development of Purkinje neurons in a dose- dependent manner. The length and branching of the dendrites were severely decreased by the antibody treatment. When other anti-glycoconjugate or sphingolipid monoclonal antibodies were tested, only HNK-1 monoclonal antibody that recognizes sulfoglucuronic residues in glycolipids and glycoproteins had similar but moderate inhibitory actions on the dendritic development of these neurons. In contrast to the morphological alterations observed in Purkinje cells, other cerebellar cells including granule neurons appear to be almost normal following the antibody treatment. These observations lead to the possibility that GD1α ganglioside has a role in the development of Purkinje cell dendrites.

Original languageEnglish
Pages (from-to)73-83
Number of pages11
JournalJournal of Neuroscience Research
Volume44
Issue number1
DOIs
Publication statusPublished - Apr 22 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

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