Restriction fragment length polymorphism analysis and random amplified polymorphic DNA analysis of Campylobacter jejuni strains isolated from patients with Guillain-Barre syndrome

Shuji Fujimoto, Ban Mishu Allos, Naoaki Misawa, Charlotte M. Patton, Martin J. Blaser

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Campylobacter jejuni serotype O19 strains associated with the Guillain- Barre syndrome (GBS) and other strains were examined by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction products of the flaA genes and by random amplified polymorphic DNA (RAPD) analysis. RFLP analysis showed that regardless of LIO serotype, geographic origins, or association with GBS, the O19 isolates shared an identical digestion pattern by each of four restriction endonucleases, DdeI, MboI, MseI; and AluI. In contrast, among C. jejuni O1 or O2 strains, RFLP patterns were different even among strains of the same LIO serotype. The results of the RAPD analysis were consistent with the flaA RFLP data. These data indicate that all of the O19 strains that were tested were closely related to one another whether they were or were not associated with GBS.

Original languageEnglish
Pages (from-to)1105-1108
Number of pages4
JournalJournal of Infectious Diseases
Volume176
Issue number4
DOIs
Publication statusPublished - Jan 1 1997

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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