Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation

Yoshinori Kawabe, Tsutomu Naka, Hiroyuki Komatsu, Ken Ichi Nishijima, Shinji Iijima, Masamichi Kamihira

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.

Original languageEnglish
Pages (from-to)598-601
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume106
Issue number6
DOIs
Publication statusPublished - Dec 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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