Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation

Kawabe Yoshinori, Tsutomu Naka, Hiroyuki Komatsu, Ken Ichi Nishijima, Shinji Iijima, Masamichi Kamihira

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.

Original languageEnglish
Pages (from-to)598-601
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume106
Issue number6
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Blood Circulation
Gonads
Hemodynamics
Germ Cells
Chickens
Embryonic Structures
Genes
Gene transfer
Transgenes
Blood
Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation. / Yoshinori, Kawabe; Naka, Tsutomu; Komatsu, Hiroyuki; Nishijima, Ken Ichi; Iijima, Shinji; Kamihira, Masamichi.

In: Journal of Bioscience and Bioengineering, Vol. 106, No. 6, 01.12.2008, p. 598-601.

Research output: Contribution to journalArticle

Yoshinori, Kawabe ; Naka, Tsutomu ; Komatsu, Hiroyuki ; Nishijima, Ken Ichi ; Iijima, Shinji ; Kamihira, Masamichi. / Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation. In: Journal of Bioscience and Bioengineering. 2008 ; Vol. 106, No. 6. pp. 598-601.
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