TY - GEN
T1 - Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene
AU - Fatchiyah,
AU - Zubair, M.
AU - Morohashi, Ken-Ichirou
PY - 2009/12/1
Y1 - 2009/12/1
N2 - The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.
AB - The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.
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U2 - 10.1007/978-3-540-92841-6_477
DO - 10.1007/978-3-540-92841-6_477
M3 - Conference contribution
AN - SCOPUS:84891953982
SN - 9783540928409
T3 - IFMBE Proceedings
SP - 1920
EP - 1923
BT - 13th International Conference on Biomedical Engineering - ICBME 2008
T2 - 13th International Conference on Biomedical Engineering, ICBME 2008
Y2 - 3 December 2008 through 6 December 2008
ER -