Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene

Fatchiyah, M. Zubair, Ken-Ichirou Morohashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages1920-1923
Number of pages4
DOIs
Publication statusPublished - Dec 1 2009
Event13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
Duration: Dec 3 2008Dec 6 2008

Publication series

NameIFMBE Proceedings
Volume23
ISSN (Print)1680-0737

Other

Other13th International Conference on Biomedical Engineering, ICBME 2008
CountrySingapore
Period12/3/0812/6/08

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All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Bioengineering

Cite this

Fatchiyah, Zubair, M., & Morohashi, K-I. (2009). Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene. In 13th International Conference on Biomedical Engineering - ICBME 2008 (pp. 1920-1923). (IFMBE Proceedings; Vol. 23). https://doi.org/10.1007/978-3-540-92841-6_477