Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers

Masaki Kurogochi, Maho Amano, Masataka Fumoto, Akio Takimoto, Hirosato Kondo, Shin Ichiro Nishimura

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

(Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).

Original languageEnglish
Pages (from-to)8808-8813
Number of pages6
JournalAngewandte Chemie - International Edition
Volume46
Issue number46
DOIs
Publication statusPublished - Dec 10 2007
Externally publishedYes

Fingerprint

Glycopeptides
Biomarkers
N-Acetylneuraminic Acid
Acetylglucosamine
Acids
Schematic diagrams
Mannose
Galactose
Glucosamine
Polymers
Throughput
Oxidation
N-glycolylneuraminic acid

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers. / Kurogochi, Masaki; Amano, Maho; Fumoto, Masataka; Takimoto, Akio; Kondo, Hirosato; Nishimura, Shin Ichiro.

In: Angewandte Chemie - International Edition, Vol. 46, No. 46, 10.12.2007, p. 8808-8813.

Research output: Contribution to journalArticle

Kurogochi, Masaki ; Amano, Maho ; Fumoto, Masataka ; Takimoto, Akio ; Kondo, Hirosato ; Nishimura, Shin Ichiro. / Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers. In: Angewandte Chemie - International Edition. 2007 ; Vol. 46, No. 46. pp. 8808-8813.
@article{d38cdff83c63492faa6b4084a4bb1f42,
title = "Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers",
abstract = "(Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).",
author = "Masaki Kurogochi and Maho Amano and Masataka Fumoto and Akio Takimoto and Hirosato Kondo and Nishimura, {Shin Ichiro}",
year = "2007",
month = "12",
day = "10",
doi = "10.1002/anie.200702919",
language = "English",
volume = "46",
pages = "8808--8813",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "46",

}

TY - JOUR

T1 - Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers

AU - Kurogochi, Masaki

AU - Amano, Maho

AU - Fumoto, Masataka

AU - Takimoto, Akio

AU - Kondo, Hirosato

AU - Nishimura, Shin Ichiro

PY - 2007/12/10

Y1 - 2007/12/10

N2 - (Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).

AB - (Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).

UR - http://www.scopus.com/inward/record.url?scp=36749040214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36749040214&partnerID=8YFLogxK

U2 - 10.1002/anie.200702919

DO - 10.1002/anie.200702919

M3 - Article

C2 - 17943939

AN - SCOPUS:36749040214

VL - 46

SP - 8808

EP - 8813

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 46

ER -