Abstract
(Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).
| Original language | English |
|---|---|
| Pages (from-to) | 8808-8813 |
| Number of pages | 6 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 46 |
| Issue number | 46 |
| DOIs | |
| Publication status | Published - Dec 10 2007 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Catalysis
- Chemistry(all)
Cite this
Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers. / Kurogochi, Masaki; Amano, Maho; Fumoto, Masataka; Takimoto, Akio; Kondo, Hirosato; Nishimura, Shin Ichiro.
In: Angewandte Chemie - International Edition, Vol. 46, No. 46, 10.12.2007, p. 8808-8813.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers
AU - Kurogochi, Masaki
AU - Amano, Maho
AU - Fumoto, Masataka
AU - Takimoto, Akio
AU - Kondo, Hirosato
AU - Nishimura, Shin Ichiro
PY - 2007/12/10
Y1 - 2007/12/10
N2 - (Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).
AB - (Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).
UR - http://www.scopus.com/inward/record.url?scp=36749040214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36749040214&partnerID=8YFLogxK
U2 - 10.1002/anie.200702919
DO - 10.1002/anie.200702919
M3 - Article
C2 - 17943939
AN - SCOPUS:36749040214
VL - 46
SP - 8808
EP - 8813
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 46
ER -