Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers

Masaki Kurogochi, Maho Amano, Masataka Fumoto, Akio Takimoto, Hirosato Kondo, Shin Ichiro Nishimura

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

(Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).

Original languageEnglish
Pages (from-to)8808-8813
Number of pages6
JournalAngewandte Chemie - International Edition
Volume46
Issue number46
DOIs
Publication statusPublished - Dec 10 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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