TY - JOUR
T1 - Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers
AU - Kurogochi, Masaki
AU - Amano, Maho
AU - Fumoto, Masataka
AU - Takimoto, Akio
AU - Kondo, Hirosato
AU - Nishimura, Shin Ichiro
PY - 2007/12/10
Y1 - 2007/12/10
N2 - (Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).
AB - (Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).
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U2 - 10.1002/anie.200702919
DO - 10.1002/anie.200702919
M3 - Article
C2 - 17943939
AN - SCOPUS:36749040214
VL - 46
SP - 8808
EP - 8813
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 46
ER -