Reverse gyrase is not a prerequisite for hyperthermophilic life

Haruyuki Atomi, Rie Matsumi, Tadayuki Imanaka

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90°C. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.

Original languageEnglish
Pages (from-to)4829-4833
Number of pages5
JournalJournal of bacteriology
Volume186
Issue number14
DOIs
Publication statusPublished - Jul 1 2004

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Thermococcus
Archaea
Genes
Phenotype
Temperature
DNA reverse gyrase
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Reverse gyrase is not a prerequisite for hyperthermophilic life. / Atomi, Haruyuki; Matsumi, Rie; Imanaka, Tadayuki.

In: Journal of bacteriology, Vol. 186, No. 14, 01.07.2004, p. 4829-4833.

Research output: Contribution to journalArticle

Atomi, Haruyuki ; Matsumi, Rie ; Imanaka, Tadayuki. / Reverse gyrase is not a prerequisite for hyperthermophilic life. In: Journal of bacteriology. 2004 ; Vol. 186, No. 14. pp. 4829-4833.
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