Reverse gyrase is not a prerequisite for hyperthermophilic life

Haruyuki Atomi, Rie Matsumi, Tadayuki Imanaka

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)

Abstract

We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90°C. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.

Original languageEnglish
Pages (from-to)4829-4833
Number of pages5
JournalJournal of bacteriology
Volume186
Issue number14
DOIs
Publication statusPublished - Jul 2004

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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