Reversed micelles as novel protein refolding media

Masahiro Goto, Tsutomu Ono, Shintaro Furusaki

Research output: Contribution to journalArticle

Abstract

Refolding of denatured RNase A was conducted by a nanostructural surfactant-based molecular assembly formulated with sodium di-2-ethylhexyl sulfosuccinate (AOT) in isooctane. In the novel refolding technique, a solid-liquid extraction was utilized as an alternative to the ordinary protein extraction by reversed micelles based on a liquid-liquid extraction. The effects of operational parameters such as concentration of AOT, Wo (=[H2O]/[AOT]), and pH were examined on the solubilization of denatured proteins into a reversed micellar solution. The typically denatured form of the protein being isolated is reactivated due to its refolding ability within the confines of the reversed micelle medium. A complete renaturation of RNase A was accomplished by adjusting the composition of the redox reagent even at a high protein concentration in which protein aggregation would usually occur in aqueous media. In the final step, the renatured RNase A was effectively recovered from the reversed micellar solution without the loss of the enzymatic activity.

Original languageEnglish
Pages (from-to)374-383
Number of pages10
JournalACS Symposium Series
Volume740
Publication statusPublished - 1999

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Micelles
Pancreatic Ribonuclease
Proteins
Liquids
Surface-Active Agents
Surface active agents
Agglomeration
Sodium
Chemical analysis
Ribonucleases

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Goto, M., Ono, T., & Furusaki, S. (1999). Reversed micelles as novel protein refolding media. ACS Symposium Series, 740, 374-383.

Reversed micelles as novel protein refolding media. / Goto, Masahiro; Ono, Tsutomu; Furusaki, Shintaro.

In: ACS Symposium Series, Vol. 740, 1999, p. 374-383.

Research output: Contribution to journalArticle

Goto, M, Ono, T & Furusaki, S 1999, 'Reversed micelles as novel protein refolding media', ACS Symposium Series, vol. 740, pp. 374-383.
Goto, Masahiro ; Ono, Tsutomu ; Furusaki, Shintaro. / Reversed micelles as novel protein refolding media. In: ACS Symposium Series. 1999 ; Vol. 740. pp. 374-383.
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