Reversed micelles recognize an active protein

Masahiro Goto, Tsutomu Ono, Fumiyuki Nakashio, T. Alan Hatton

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The extraction behavior of native and heated-denatured α-chymotrypsin has been investigated with two different reversed micellar systems. A large difference in the degree of extraction was observed for the native relative to the denatured α-chymotrypsin. In particular, mixed reversed micelles formulated with DOLPA (dioleyl phosphoric acid) and AOT show a high selectivity for the active α-chymotrypsin.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalBiotechnology Techniques
Volume10
Issue number3
DOIs
Publication statusPublished - Jan 1 1996

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Chymotrypsin
Micelles
Proteins
Phosphoric acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Reversed micelles recognize an active protein. / Goto, Masahiro; Ono, Tsutomu; Nakashio, Fumiyuki; Hatton, T. Alan.

In: Biotechnology Techniques, Vol. 10, No. 3, 01.01.1996, p. 141-144.

Research output: Contribution to journalArticle

Goto, M, Ono, T, Nakashio, F & Hatton, TA 1996, 'Reversed micelles recognize an active protein', Biotechnology Techniques, vol. 10, no. 3, pp. 141-144. https://doi.org/10.1007/BF00158935
Goto, Masahiro ; Ono, Tsutomu ; Nakashio, Fumiyuki ; Hatton, T. Alan. / Reversed micelles recognize an active protein. In: Biotechnology Techniques. 1996 ; Vol. 10, No. 3. pp. 141-144.
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