TY - JOUR
T1 - Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers
AU - Shimada, Naohiko
AU - Saito, Minako
AU - Shukuri, Sayaka
AU - Kuroyanagi, Sotaro
AU - Kuboki, Thasaneeya
AU - Kidoaki, Satoru
AU - Nagai, Takeharu
AU - Maruyama, Atsushi
N1 - Funding Information:
This work was financially supported by a Grant-in-Aid for Scientific Research on Innovative Areas "Molecular Robotics" (No. 15H00804), "Nanomedicine Molecular Science" (No. 2306) and the Cooperative Research Program of "Network Joint Research Center for Materials and Devices" from the Ministry of Education, Culture, Sports, Science and Technology, by Center of Innovation (COI) Program, Japan Science and Technology Agency (JST), and by KAKENHI (No. 15H01807, 25350552) from Japan Society for the Promotion of Science.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/11/23
Y1 - 2016/11/23
N2 - Multicellular spheroids have been studied in the fields of oncology, stem cell biology, and tissue engineering. In this study, we found a new polymer material for thermo-controlled spheroid/monolayer cell culture switching. The polymers that have pendant ureido groups (ureido polymers) exhibited upper critical solution temperature-type phase separation behavior. Cells in monolayer culture were converted to spheroids by the addition of ureido polymers below phase separation temperature (Tp). Time-lapse observations indicated that cells began to migrate and aggregate to form the spheroids to avoid contact with phase-separated polymer (coacervates) on the surface of the culture dish. We supposed that the coacervates seemingly suppressed interaction between cell and the dish surface or extracellular matrices. By increasing culture temperature above Tp, the spheroids began to collapse into a monolayer of cells due to dissolution of the coacervates. These results indicated that cell morphology could be repeatedly switched by changing the culture temperature in the presence of ureido polymers.
AB - Multicellular spheroids have been studied in the fields of oncology, stem cell biology, and tissue engineering. In this study, we found a new polymer material for thermo-controlled spheroid/monolayer cell culture switching. The polymers that have pendant ureido groups (ureido polymers) exhibited upper critical solution temperature-type phase separation behavior. Cells in monolayer culture were converted to spheroids by the addition of ureido polymers below phase separation temperature (Tp). Time-lapse observations indicated that cells began to migrate and aggregate to form the spheroids to avoid contact with phase-separated polymer (coacervates) on the surface of the culture dish. We supposed that the coacervates seemingly suppressed interaction between cell and the dish surface or extracellular matrices. By increasing culture temperature above Tp, the spheroids began to collapse into a monolayer of cells due to dissolution of the coacervates. These results indicated that cell morphology could be repeatedly switched by changing the culture temperature in the presence of ureido polymers.
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U2 - 10.1021/acsami.6b07614
DO - 10.1021/acsami.6b07614
M3 - Article
C2 - 27802011
AN - SCOPUS:84999176903
VL - 8
SP - 31524
EP - 31529
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 46
ER -