Abstract
Influences of heat stress on epicotyls of pea (Pisum sativum L.) plants were investigated by using non-destructive method. NMR spin-lattice relaxation time (T 1), indicating molecular dynamics of water, was determined in tall type, cv. Alaska and dwarf type, cv. Progress No. 9 seedlings grown under light condition. In no-elongation zone for both fresh cultivars a rapid decrease in T 1 occurred in the tissues exposed to 20-30°C while rapid increase followed by gradual decrease occurred in T 1 for the tissues exposed to 20-40°C during a heat-cool cycle. On the other hand, in heat-denatured dead tissues, T 1 linearly corresponded. Further, when pea plants were heated for 5 h at 20-30°C or 20-40°C thermal hysteresis changes in T 1 corresponding temperature in the subsequent heat-cool cycles was notably different. Epicotyls exposed to 30°C treatments did not show temperature dependency while those of the tissues exposed to 40°C indicated temperature dependency. After the subsequent heat-cool cycles, the former was alive, the latter indicated tissue necrosis. Therefore, the change in T 1 of epicotyls observed within 1 h was considered a fast adaptation or mortality in cells to heat stress, and a temperature dependency of T 1 in the subsequent heat-cool cycles clearly reflected tissue viability in pea epicotyls. In conclusion, T 1 on thermal response can be used as an indicator of reversible or irreversible injury in an intact plant.
| Original language | English |
|---|---|
| Pages (from-to) | 39-47 |
| Number of pages | 9 |
| Journal | Journal of the Faculty of Agriculture, Kyushu University |
| Volume | 49 |
| Issue number | 1 |
| Publication status | Published - Feb 2004 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences (miscellaneous)
Cite this
Reversible or irreversible change of molecular dynamics of water in pea seedlings exposed to heat stress. / Iwaya-Inoue, Mari; Motooka, Kei; Nakamoto, Hiroaki; Fukuyama, Masataka.
In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 49, No. 1, 02.2004, p. 39-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reversible or irreversible change of molecular dynamics of water in pea seedlings exposed to heat stress
AU - Iwaya-Inoue, Mari
AU - Motooka, Kei
AU - Nakamoto, Hiroaki
AU - Fukuyama, Masataka
PY - 2004/2
Y1 - 2004/2
N2 - Influences of heat stress on epicotyls of pea (Pisum sativum L.) plants were investigated by using non-destructive method. NMR spin-lattice relaxation time (T 1), indicating molecular dynamics of water, was determined in tall type, cv. Alaska and dwarf type, cv. Progress No. 9 seedlings grown under light condition. In no-elongation zone for both fresh cultivars a rapid decrease in T 1 occurred in the tissues exposed to 20-30°C while rapid increase followed by gradual decrease occurred in T 1 for the tissues exposed to 20-40°C during a heat-cool cycle. On the other hand, in heat-denatured dead tissues, T 1 linearly corresponded. Further, when pea plants were heated for 5 h at 20-30°C or 20-40°C thermal hysteresis changes in T 1 corresponding temperature in the subsequent heat-cool cycles was notably different. Epicotyls exposed to 30°C treatments did not show temperature dependency while those of the tissues exposed to 40°C indicated temperature dependency. After the subsequent heat-cool cycles, the former was alive, the latter indicated tissue necrosis. Therefore, the change in T 1 of epicotyls observed within 1 h was considered a fast adaptation or mortality in cells to heat stress, and a temperature dependency of T 1 in the subsequent heat-cool cycles clearly reflected tissue viability in pea epicotyls. In conclusion, T 1 on thermal response can be used as an indicator of reversible or irreversible injury in an intact plant.
AB - Influences of heat stress on epicotyls of pea (Pisum sativum L.) plants were investigated by using non-destructive method. NMR spin-lattice relaxation time (T 1), indicating molecular dynamics of water, was determined in tall type, cv. Alaska and dwarf type, cv. Progress No. 9 seedlings grown under light condition. In no-elongation zone for both fresh cultivars a rapid decrease in T 1 occurred in the tissues exposed to 20-30°C while rapid increase followed by gradual decrease occurred in T 1 for the tissues exposed to 20-40°C during a heat-cool cycle. On the other hand, in heat-denatured dead tissues, T 1 linearly corresponded. Further, when pea plants were heated for 5 h at 20-30°C or 20-40°C thermal hysteresis changes in T 1 corresponding temperature in the subsequent heat-cool cycles was notably different. Epicotyls exposed to 30°C treatments did not show temperature dependency while those of the tissues exposed to 40°C indicated temperature dependency. After the subsequent heat-cool cycles, the former was alive, the latter indicated tissue necrosis. Therefore, the change in T 1 of epicotyls observed within 1 h was considered a fast adaptation or mortality in cells to heat stress, and a temperature dependency of T 1 in the subsequent heat-cool cycles clearly reflected tissue viability in pea epicotyls. In conclusion, T 1 on thermal response can be used as an indicator of reversible or irreversible injury in an intact plant.
UR - http://www.scopus.com/inward/record.url?scp=2442599355&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442599355&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:2442599355
VL - 49
SP - 39
EP - 47
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 1
ER -