Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

Ryosuke Kawagoe, Ippei Takashima, Shohei Uchinomiya, Akio Ojida

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change. The reversible nature of this reaction allows us to detect increases and decreases of hydropersulfide levels in a real-time manner. The probe fluorometrically sensed highly reactive hydropersulfides, such as H2S2 and Cys-SSH, while the fluorescence response to biologically abundant cysteine and glutathione was negligible. Taking advantage of the reversible and selective sensing properties, this probe was successfully applied to the ratiometric imaging of concentration dynamics of endogenously produced hydropersulfides in living cells.

Original languageEnglish
Pages (from-to)1134-1140
Number of pages7
JournalChemical Science
Volume8
Issue number2
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Fluorescent Dyes
Pyronine
Cells
Cell signaling
Mammals
Sulfur
Glutathione
Cysteine
Fluorescence
Imaging techniques
Fluorescence Resonance Energy Transfer

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe. / Kawagoe, Ryosuke; Takashima, Ippei; Uchinomiya, Shohei; Ojida, Akio.

In: Chemical Science, Vol. 8, No. 2, 01.01.2017, p. 1134-1140.

Research output: Contribution to journalArticle

@article{4323988adf56468d8afadbeaf91b0763,
title = "Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe",
abstract = "Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change. The reversible nature of this reaction allows us to detect increases and decreases of hydropersulfide levels in a real-time manner. The probe fluorometrically sensed highly reactive hydropersulfides, such as H2S2 and Cys-SSH, while the fluorescence response to biologically abundant cysteine and glutathione was negligible. Taking advantage of the reversible and selective sensing properties, this probe was successfully applied to the ratiometric imaging of concentration dynamics of endogenously produced hydropersulfides in living cells.",
author = "Ryosuke Kawagoe and Ippei Takashima and Shohei Uchinomiya and Akio Ojida",
year = "2017",
month = "1",
day = "1",
doi = "10.1039/C6SC03856E",
language = "English",
volume = "8",
pages = "1134--1140",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "2",

}

TY - JOUR

T1 - Reversible ratiometric detection of highly reactive hydropersulfides using a FRET-based dual emission fluorescent probe

AU - Kawagoe, Ryosuke

AU - Takashima, Ippei

AU - Uchinomiya, Shohei

AU - Ojida, Akio

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change. The reversible nature of this reaction allows us to detect increases and decreases of hydropersulfide levels in a real-time manner. The probe fluorometrically sensed highly reactive hydropersulfides, such as H2S2 and Cys-SSH, while the fluorescence response to biologically abundant cysteine and glutathione was negligible. Taking advantage of the reversible and selective sensing properties, this probe was successfully applied to the ratiometric imaging of concentration dynamics of endogenously produced hydropersulfides in living cells.

AB - Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change. The reversible nature of this reaction allows us to detect increases and decreases of hydropersulfide levels in a real-time manner. The probe fluorometrically sensed highly reactive hydropersulfides, such as H2S2 and Cys-SSH, while the fluorescence response to biologically abundant cysteine and glutathione was negligible. Taking advantage of the reversible and selective sensing properties, this probe was successfully applied to the ratiometric imaging of concentration dynamics of endogenously produced hydropersulfides in living cells.

UR - http://www.scopus.com/inward/record.url?scp=85010987640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85010987640&partnerID=8YFLogxK

U2 - 10.1039/C6SC03856E

DO - 10.1039/C6SC03856E

M3 - Article

VL - 8

SP - 1134

EP - 1140

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 2

ER -