Revised model for Enterococcus faecalis fsr quorum-sensing system

The small open reading frame fsrD encodes the gelatinase biosynthesis-activating pheromone propeptide corresponding to staphylococcal AgrD

Jiro Nakayama, Shengmin Chen, Nozomi Oyama, Kenzo Nishiguchi, Essam A. Azab, Emi Tanaka, Reiko Kariyama, Kenji Sonomoto

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Gelatinase biosynthesis-activating pheromone (GBAP) is an autoinducing peptide involved in Enterococcus faecalis fsr quorum sensing, and its 11-amino-acid sequence has been identified in the C-terminal region of the 242-residue deduced fsrB product (J. Nakayama et al., Mol. Microbiol. 41:145-154, 2001). In this study, however, we demonstrated the existence of fsrD, encoding the GBAP propeptide, which is in frame with fsrB but is translated independently of fsrB. It was also demonstrated that FsrB′, an FsrD segment-truncated FsrB, functions as a cysteine protease-like processing enzyme to generate GBAP from FsrD. This revised model is consistent with the staphylococcal agr system.

Original languageEnglish
Pages (from-to)8321-8326
Number of pages6
JournalJournal of bacteriology
Volume188
Issue number23
DOIs
Publication statusPublished - Dec 1 2006

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Quorum Sensing
Enterococcus faecalis
Open Reading Frames
Cysteine Proteases
Amino Acid Sequence
Peptides
Enzymes
gelatinase biosynthesis-activating pheromone

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Revised model for Enterococcus faecalis fsr quorum-sensing system : The small open reading frame fsrD encodes the gelatinase biosynthesis-activating pheromone propeptide corresponding to staphylococcal AgrD. / Nakayama, Jiro; Chen, Shengmin; Oyama, Nozomi; Nishiguchi, Kenzo; Azab, Essam A.; Tanaka, Emi; Kariyama, Reiko; Sonomoto, Kenji.

In: Journal of bacteriology, Vol. 188, No. 23, 01.12.2006, p. 8321-8326.

Research output: Contribution to journalArticle

Nakayama, Jiro ; Chen, Shengmin ; Oyama, Nozomi ; Nishiguchi, Kenzo ; Azab, Essam A. ; Tanaka, Emi ; Kariyama, Reiko ; Sonomoto, Kenji. / Revised model for Enterococcus faecalis fsr quorum-sensing system : The small open reading frame fsrD encodes the gelatinase biosynthesis-activating pheromone propeptide corresponding to staphylococcal AgrD. In: Journal of bacteriology. 2006 ; Vol. 188, No. 23. pp. 8321-8326.
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abstract = "Gelatinase biosynthesis-activating pheromone (GBAP) is an autoinducing peptide involved in Enterococcus faecalis fsr quorum sensing, and its 11-amino-acid sequence has been identified in the C-terminal region of the 242-residue deduced fsrB product (J. Nakayama et al., Mol. Microbiol. 41:145-154, 2001). In this study, however, we demonstrated the existence of fsrD, encoding the GBAP propeptide, which is in frame with fsrB but is translated independently of fsrB. It was also demonstrated that FsrB′, an FsrD segment-truncated FsrB, functions as a cysteine protease-like processing enzyme to generate GBAP from FsrD. This revised model is consistent with the staphylococcal agr system.",
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AU - Azab, Essam A.

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