RFLP framework map using recombinant inbred lines in rice

Hiroshi Tsunematsu, Atsushi Yoshimura, Yoshiaki Harushima, Yoshiaki Nagamura, Nori Kurata, Masahiro Yano, Takuji Sasaki, Nobuo Iwata

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Recombinant inbred (RI) lines of rice (Oryza sativa L.) were developed from a cross of Asominori x IR24 by the single seed descent method. Seventy- one RI lines of the F6 and F7 generations were used for RFLP mapping. RFLP framework map constructed using the RI lines covered a distance of 1275cM containing 375 markers. All the twelve linkage groups were assigned to their respective chromosomes. The RI lines showed a distorted segregation in some regions of chromosomes 1, 3, 6, 11 and 12. Heterozygous loci in the lines still remained. Average frequency of the heterozygous region was 3.6% with a range of 0% - 19.3% in F6 and 1.9% with a range of 0% - 5.5% in F7. The RFLP map corresponded well to the previous maps constructed using F2 populations (Saito et al. 1991, Kurata et al. 1994) and the two previous maps were integrated in detail. The RFLP framework map and RI lines developed in this study will be useful for further accumulation of molecular markers and analysis of quantitative trait loci.

Original languageEnglish
Pages (from-to)279-284
Number of pages6
JournalBreeding Science
Volume46
Issue number3
DOIs
Publication statusPublished - Sep 1996

Fingerprint

Restriction Fragment Length Polymorphisms
inbred lines
restriction fragment length polymorphism
rice
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 1
Quantitative Trait Loci
chromosomes
Seeds
linkage groups
Chromosomes
quantitative trait loci
Oryza sativa
Oryza
loci
genetic markers
Population
seeds
methodology

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Tsunematsu, H., Yoshimura, A., Harushima, Y., Nagamura, Y., Kurata, N., Yano, M., ... Iwata, N. (1996). RFLP framework map using recombinant inbred lines in rice. Breeding Science, 46(3), 279-284. https://doi.org/10.1270/jsbbs1951.46.279

RFLP framework map using recombinant inbred lines in rice. / Tsunematsu, Hiroshi; Yoshimura, Atsushi; Harushima, Yoshiaki; Nagamura, Yoshiaki; Kurata, Nori; Yano, Masahiro; Sasaki, Takuji; Iwata, Nobuo.

In: Breeding Science, Vol. 46, No. 3, 09.1996, p. 279-284.

Research output: Contribution to journalArticle

Tsunematsu, H, Yoshimura, A, Harushima, Y, Nagamura, Y, Kurata, N, Yano, M, Sasaki, T & Iwata, N 1996, 'RFLP framework map using recombinant inbred lines in rice', Breeding Science, vol. 46, no. 3, pp. 279-284. https://doi.org/10.1270/jsbbs1951.46.279
Tsunematsu H, Yoshimura A, Harushima Y, Nagamura Y, Kurata N, Yano M et al. RFLP framework map using recombinant inbred lines in rice. Breeding Science. 1996 Sep;46(3):279-284. https://doi.org/10.1270/jsbbs1951.46.279
Tsunematsu, Hiroshi ; Yoshimura, Atsushi ; Harushima, Yoshiaki ; Nagamura, Yoshiaki ; Kurata, Nori ; Yano, Masahiro ; Sasaki, Takuji ; Iwata, Nobuo. / RFLP framework map using recombinant inbred lines in rice. In: Breeding Science. 1996 ; Vol. 46, No. 3. pp. 279-284.
@article{918c4313c3844689a5ed6d508a16417f,
title = "RFLP framework map using recombinant inbred lines in rice",
abstract = "Recombinant inbred (RI) lines of rice (Oryza sativa L.) were developed from a cross of Asominori x IR24 by the single seed descent method. Seventy- one RI lines of the F6 and F7 generations were used for RFLP mapping. RFLP framework map constructed using the RI lines covered a distance of 1275cM containing 375 markers. All the twelve linkage groups were assigned to their respective chromosomes. The RI lines showed a distorted segregation in some regions of chromosomes 1, 3, 6, 11 and 12. Heterozygous loci in the lines still remained. Average frequency of the heterozygous region was 3.6{\%} with a range of 0{\%} - 19.3{\%} in F6 and 1.9{\%} with a range of 0{\%} - 5.5{\%} in F7. The RFLP map corresponded well to the previous maps constructed using F2 populations (Saito et al. 1991, Kurata et al. 1994) and the two previous maps were integrated in detail. The RFLP framework map and RI lines developed in this study will be useful for further accumulation of molecular markers and analysis of quantitative trait loci.",
author = "Hiroshi Tsunematsu and Atsushi Yoshimura and Yoshiaki Harushima and Yoshiaki Nagamura and Nori Kurata and Masahiro Yano and Takuji Sasaki and Nobuo Iwata",
year = "1996",
month = "9",
doi = "10.1270/jsbbs1951.46.279",
language = "English",
volume = "46",
pages = "279--284",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "3",

}

TY - JOUR

T1 - RFLP framework map using recombinant inbred lines in rice

AU - Tsunematsu, Hiroshi

AU - Yoshimura, Atsushi

AU - Harushima, Yoshiaki

AU - Nagamura, Yoshiaki

AU - Kurata, Nori

AU - Yano, Masahiro

AU - Sasaki, Takuji

AU - Iwata, Nobuo

PY - 1996/9

Y1 - 1996/9

N2 - Recombinant inbred (RI) lines of rice (Oryza sativa L.) were developed from a cross of Asominori x IR24 by the single seed descent method. Seventy- one RI lines of the F6 and F7 generations were used for RFLP mapping. RFLP framework map constructed using the RI lines covered a distance of 1275cM containing 375 markers. All the twelve linkage groups were assigned to their respective chromosomes. The RI lines showed a distorted segregation in some regions of chromosomes 1, 3, 6, 11 and 12. Heterozygous loci in the lines still remained. Average frequency of the heterozygous region was 3.6% with a range of 0% - 19.3% in F6 and 1.9% with a range of 0% - 5.5% in F7. The RFLP map corresponded well to the previous maps constructed using F2 populations (Saito et al. 1991, Kurata et al. 1994) and the two previous maps were integrated in detail. The RFLP framework map and RI lines developed in this study will be useful for further accumulation of molecular markers and analysis of quantitative trait loci.

AB - Recombinant inbred (RI) lines of rice (Oryza sativa L.) were developed from a cross of Asominori x IR24 by the single seed descent method. Seventy- one RI lines of the F6 and F7 generations were used for RFLP mapping. RFLP framework map constructed using the RI lines covered a distance of 1275cM containing 375 markers. All the twelve linkage groups were assigned to their respective chromosomes. The RI lines showed a distorted segregation in some regions of chromosomes 1, 3, 6, 11 and 12. Heterozygous loci in the lines still remained. Average frequency of the heterozygous region was 3.6% with a range of 0% - 19.3% in F6 and 1.9% with a range of 0% - 5.5% in F7. The RFLP map corresponded well to the previous maps constructed using F2 populations (Saito et al. 1991, Kurata et al. 1994) and the two previous maps were integrated in detail. The RFLP framework map and RI lines developed in this study will be useful for further accumulation of molecular markers and analysis of quantitative trait loci.

UR - http://www.scopus.com/inward/record.url?scp=0029911092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029911092&partnerID=8YFLogxK

U2 - 10.1270/jsbbs1951.46.279

DO - 10.1270/jsbbs1951.46.279

M3 - Article

AN - SCOPUS:0029911092

VL - 46

SP - 279

EP - 284

JO - Breeding Science

JF - Breeding Science

SN - 1344-7610

IS - 3

ER -