Rho/Rho-Kinase Pathway in the Brainstem Contributes to Hypertension Caused by Chronic Nitric Oxide Synthase Inhibition

Koji Ito, Yoshitaka Hirooka, Takuya Kishi, Yoshikuni Kimura, Kozo Kaibuchi, Hiroaki Shimokawa, Akira Takeshita

Research output: Contribution to journalArticle

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Abstract

Central nervous system mechanisms are involved in hypertension caused by chronic inhibition of nitric oxide (NO) synthesis. Chronic inhibition of NO synthesis might also activate the Rho/Rho-kinase pathway in the vasculature. We recently demonstrated that activation of the Rho/Rho-kinase pathway in the nucleus tractus solitarii (NTS) contributes to hypertensive mechanisms in spontaneously hypertensive rats. The aim of the present study was to determine whether activation of this pathway also contributes to neurogenic hypertensive mechanisms caused by chronic NO synthesis inhibition. The NO synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME) was administered to Wistar-Kyoto rats in their drinking water (1 mg/mL) for 2 weeks. Bilateral microinjection of Y-27632, a specific Rho-kinase inhibitor, into the NTS elicited decreases in arterial pressure, heart rate, and renal sympathetic nerve activity in control rats and L-NAME-treated rats. The magnitude of the decrease, however, was significantly greater in L-NAME-treated than in control rats. In another group of rats, the specific Rho-kinase inhibitor, Y-27632, was administered intracisternally for 2 weeks with a mini-osmotic pump from the beginning of treatment with L-NAME. Y-27632 co-treatment significantly attenuated the increase in arterial pressure. Furthermore, the expression level of membranous RhoA and phosphorylation of the target proteins of Rho-kinase, the ERM (ezrin, radixin, moesin) family members, was significantly greater in L-NAME-treated rats than in control rats. These results indicate that activation of the Rho/Rho-kinase pathway in the NTS contributes to neurogenic hypertension caused by chronic NO synthase inhibition.

Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalHypertension
Volume43
Issue number2 I
DOIs
Publication statusPublished - Feb 1 2004

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rho-Associated Kinases
Nitric Oxide Synthase
NG-Nitroarginine Methyl Ester
Brain Stem
Hypertension
Solitary Nucleus
Nitric Oxide
Arterial Pressure
Inbred WKY Rats
Microinjections
Inbred SHR Rats
Drinking Water
Protein Kinases
Inhibition (Psychology)
Central Nervous System
Heart Rate
Phosphorylation
Kidney
Therapeutics
Y 27632

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Ito, K., Hirooka, Y., Kishi, T., Kimura, Y., Kaibuchi, K., Shimokawa, H., & Takeshita, A. (2004). Rho/Rho-Kinase Pathway in the Brainstem Contributes to Hypertension Caused by Chronic Nitric Oxide Synthase Inhibition. Hypertension, 43(2 I), 156-162. https://doi.org/10.1161/01.HYP.0000114602.82140.a4

Rho/Rho-Kinase Pathway in the Brainstem Contributes to Hypertension Caused by Chronic Nitric Oxide Synthase Inhibition. / Ito, Koji; Hirooka, Yoshitaka; Kishi, Takuya; Kimura, Yoshikuni; Kaibuchi, Kozo; Shimokawa, Hiroaki; Takeshita, Akira.

In: Hypertension, Vol. 43, No. 2 I, 01.02.2004, p. 156-162.

Research output: Contribution to journalArticle

Ito, K, Hirooka, Y, Kishi, T, Kimura, Y, Kaibuchi, K, Shimokawa, H & Takeshita, A 2004, 'Rho/Rho-Kinase Pathway in the Brainstem Contributes to Hypertension Caused by Chronic Nitric Oxide Synthase Inhibition', Hypertension, vol. 43, no. 2 I, pp. 156-162. https://doi.org/10.1161/01.HYP.0000114602.82140.a4
Ito, Koji ; Hirooka, Yoshitaka ; Kishi, Takuya ; Kimura, Yoshikuni ; Kaibuchi, Kozo ; Shimokawa, Hiroaki ; Takeshita, Akira. / Rho/Rho-Kinase Pathway in the Brainstem Contributes to Hypertension Caused by Chronic Nitric Oxide Synthase Inhibition. In: Hypertension. 2004 ; Vol. 43, No. 2 I. pp. 156-162.
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