Ribosomes in a stacked array: Elucidation of the step in translation elongation at which they are stalled during S-adenosyl-l-methionine-induced translation arrest of CGS1 mRNA

Yui Yamashita, Yoshitomo Kadokura, Naoyuki Sotta, Toru Fujiwara, Ichigaku Takigawa, Akiko Satake, Hitoshi Onouchi, Satoshi Naito

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: S-Adenosyl-L-methionine arrests ribosomes on CGS1 mRNA at the pre-translocation step, and ribosomes stack behind it. Results: Puromycin reactivity of stacked ribosomes was intermediate between ribosomes at the pre- and post-translocation steps. Conclusion: Ribosomes are stacked at nine-codon intervals, and the majority are stalled at an early step of translocation. Significance: This study is the first report of ribosomal states in a stacked array.

Original languageEnglish
Pages (from-to)12693-12704
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number18
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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