Ribosomes in a stacked array: Elucidation of the step in translation elongation at which they are stalled during S-adenosyl-l-methionine-induced translation arrest of CGS1 mRNA

Yui Yamashita, Yoshitomo Kadokura, Naoyuki Sotta, Toru Fujiwara, Ichigaku Takigawa, Akiko Satake, Hitoshi Onouchi, Satoshi Naito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: S-Adenosyl-L-methionine arrests ribosomes on CGS1 mRNA at the pre-translocation step, and ribosomes stack behind it. Results: Puromycin reactivity of stacked ribosomes was intermediate between ribosomes at the pre- and post-translocation steps. Conclusion: Ribosomes are stacked at nine-codon intervals, and the majority are stalled at an early step of translocation. Significance: This study is the first report of ribosomal states in a stacked array.

Original languageEnglish
Pages (from-to)12693-12704
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number18
DOIs
Publication statusPublished - Jan 1 2014

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S-Adenosylmethionine
Puromycin
Ribosomes
Methionine
Elongation
Messenger RNA
Codon

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Ribosomes in a stacked array : Elucidation of the step in translation elongation at which they are stalled during S-adenosyl-l-methionine-induced translation arrest of CGS1 mRNA. / Yamashita, Yui; Kadokura, Yoshitomo; Sotta, Naoyuki; Fujiwara, Toru; Takigawa, Ichigaku; Satake, Akiko; Onouchi, Hitoshi; Naito, Satoshi.

In: Journal of Biological Chemistry, Vol. 289, No. 18, 01.01.2014, p. 12693-12704.

Research output: Contribution to journalArticle

Yamashita, Yui ; Kadokura, Yoshitomo ; Sotta, Naoyuki ; Fujiwara, Toru ; Takigawa, Ichigaku ; Satake, Akiko ; Onouchi, Hitoshi ; Naito, Satoshi. / Ribosomes in a stacked array : Elucidation of the step in translation elongation at which they are stalled during S-adenosyl-l-methionine-induced translation arrest of CGS1 mRNA. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 18. pp. 12693-12704.
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