Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development1[w][oa]

Soo Cheul Yoo, Sung Hwan Cho, Hiroki Sugimoto, Jinjie Li, Kensuke Kusumi, Hee Jong Koh, Koh Iba, Nam Chon Paek

Research output: Contribution to journalArticle

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Abstract

The virescent3 (v3) and stripe1 (st1) mutants in rice (Oryza sativa) produce chlorotic leaves in a growth stage-dependent manner under field conditions. They are temperature-conditional mutants that produce bleached leaves at a constant 20°C or 30°C but almost green leaves under diurnal 30°C/20°C conditions. Here, we show V3 and St1, which encode the large and small subunits of ribonucleotide reductase (RNR), RNRL1, and RNRS1, respectively. RNR regulates the rate of deoxyribonucleotide production for DNA synthesis and repair. RNRL1 and RNRS1 are highly expressed in the shoot base and in young leaves, and the expression of the genes that function in plastid transcription/translation and in photosynthesis is altered in v3 and st1 mutants, indicating that a threshold activity of RNR is required for chloroplast biogenesis in developing leaves. There are additional RNR homologs in rice, RNRL2 and RNRS2, and eukaryotic RNRs comprise α2β 2 heterodimers. In yeast, RNRL1 interacts with RNRS1 (RNRL1:RNRS1) and RNRL2:RNRS2, but no interaction occurs between other combinations of the large and small subunits. The interacting activities are RNRL1:RNRS1 > RNRL1:rnrs1(st1) > rnrl1(v3):RNRS1 > rnrl1(v3):rnrs1(st1), which correlate with the degree of chlorosis for each genotype. This suggests that missense mutations in rnrl1(v3) and rnrs1 (st1) attenuate the first αβ dimerization. Moreover, wild-type plants exposed to a low concentration of an RNR inhibitor, hydroxyurea, produce chlorotic leaves without growth retardation, reminiscent of v3 and st1 mutants. We thus propose that upon insufficient activity of RNR, plastid DNA synthesis is preferentially arrested to allow nuclear genome replication in developing leaves, leading to continuous plant growth.

Original languageEnglish
Pages (from-to)388-401
Number of pages14
JournalPlant physiology
Volume150
Issue number1
DOIs
Publication statusPublished - May 1 2009

Fingerprint

ribonucleotide reductase
Ribonucleotide Reductases
Chloroplasts
chloroplasts
rice
leaves
Plastids
mutants
Growth
Deoxyribonucleotides
Hypochromic Anemia
Hydroxyurea
hydroxyurea
Photosynthesis
Dimerization
Missense Mutation
missense mutation
synthesis
dimerization
plastid DNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development1[w][oa]. / Yoo, Soo Cheul; Cho, Sung Hwan; Sugimoto, Hiroki; Li, Jinjie; Kusumi, Kensuke; Koh, Hee Jong; Iba, Koh; Paek, Nam Chon.

In: Plant physiology, Vol. 150, No. 1, 01.05.2009, p. 388-401.

Research output: Contribution to journalArticle

Yoo, Soo Cheul ; Cho, Sung Hwan ; Sugimoto, Hiroki ; Li, Jinjie ; Kusumi, Kensuke ; Koh, Hee Jong ; Iba, Koh ; Paek, Nam Chon. / Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development1[w][oa]. In: Plant physiology. 2009 ; Vol. 150, No. 1. pp. 388-401.
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abstract = "The virescent3 (v3) and stripe1 (st1) mutants in rice (Oryza sativa) produce chlorotic leaves in a growth stage-dependent manner under field conditions. They are temperature-conditional mutants that produce bleached leaves at a constant 20°C or 30°C but almost green leaves under diurnal 30°C/20°C conditions. Here, we show V3 and St1, which encode the large and small subunits of ribonucleotide reductase (RNR), RNRL1, and RNRS1, respectively. RNR regulates the rate of deoxyribonucleotide production for DNA synthesis and repair. RNRL1 and RNRS1 are highly expressed in the shoot base and in young leaves, and the expression of the genes that function in plastid transcription/translation and in photosynthesis is altered in v3 and st1 mutants, indicating that a threshold activity of RNR is required for chloroplast biogenesis in developing leaves. There are additional RNR homologs in rice, RNRL2 and RNRS2, and eukaryotic RNRs comprise α2β 2 heterodimers. In yeast, RNRL1 interacts with RNRS1 (RNRL1:RNRS1) and RNRL2:RNRS2, but no interaction occurs between other combinations of the large and small subunits. The interacting activities are RNRL1:RNRS1 > RNRL1:rnrs1(st1) > rnrl1(v3):RNRS1 > rnrl1(v3):rnrs1(st1), which correlate with the degree of chlorosis for each genotype. This suggests that missense mutations in rnrl1(v3) and rnrs1 (st1) attenuate the first αβ dimerization. Moreover, wild-type plants exposed to a low concentration of an RNR inhibitor, hydroxyurea, produce chlorotic leaves without growth retardation, reminiscent of v3 and st1 mutants. We thus propose that upon insufficient activity of RNR, plastid DNA synthesis is preferentially arrested to allow nuclear genome replication in developing leaves, leading to continuous plant growth.",
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AU - Sugimoto, Hiroki

AU - Li, Jinjie

AU - Kusumi, Kensuke

AU - Koh, Hee Jong

AU - Iba, Koh

AU - Paek, Nam Chon

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