TY - JOUR
T1 - Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate-protein complex
AU - Okada, Hiroki
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
N1 - Funding Information:
The present work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Technology (MEXT), Japan and a grant from the New Energy and Industrial Technology Development Organization (NEDO) of Ministry of Economy, Trade and Industry (METI), Japan. We gratefully acknowledge A. Shibata, Associate Professor, University of Tokushima, for valuable discussions.
PY - 2008/5/30
Y1 - 2008/5/30
N2 - We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10-5 cm2 V-1 s-1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.
AB - We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10-5 cm2 V-1 s-1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.
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U2 - 10.1016/j.chroma.2008.03.012
DO - 10.1016/j.chroma.2008.03.012
M3 - Article
C2 - 18430430
AN - SCOPUS:43049089322
VL - 1192
SP - 289
EP - 293
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -