Abstract
We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10-5 cm2 V-1 s-1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.
| Original language | English |
|---|---|
| Pages (from-to) | 289-293 |
| Number of pages | 5 |
| Journal | Journal of Chromatography A |
| Volume | 1192 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 30 2008 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate-protein complex. / Okada, Hiroki; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.
In: Journal of Chromatography A, Vol. 1192, No. 2, 30.05.2008, p. 289-293.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate-protein complex
AU - Okada, Hiroki
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2008/5/30
Y1 - 2008/5/30
N2 - We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10-5 cm2 V-1 s-1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.
AB - We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10-5 cm2 V-1 s-1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.
UR - http://www.scopus.com/inward/record.url?scp=43049089322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43049089322&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2008.03.012
DO - 10.1016/j.chroma.2008.03.012
M3 - Article
C2 - 18430430
AN - SCOPUS:43049089322
VL - 1192
SP - 289
EP - 293
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 2
ER -