Rna-binding protein rbp-p is required for glutelin and prolamine mrna localization in rice endosperm cells[open]

Li Tian, Hong Li Chou, Laining Zhang, Seon Kap Hwang, Shawn R. Starkenburg, Kelly A. Doroshenk, Toshihiro Kumamaru, Thomas W. Okita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.

Original languageEnglish
Pages (from-to)2529-2552
Number of pages24
JournalPlant Cell
Volume30
Issue number10
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Phenylpropanolamine
Endosperm
glutelins
RNA-Binding Proteins
Glutens
endosperm
binding proteins
storage proteins
RNA
rice
Messenger RNA
Proteins
cells
mutants
RNA-binding proteins
dwarfing
protein-protein interactions
seed development
transcriptomics
phenotypic variation

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Tian, L., Chou, H. L., Zhang, L., Hwang, S. K., Starkenburg, S. R., Doroshenk, K. A., ... Okita, T. W. (2018). Rna-binding protein rbp-p is required for glutelin and prolamine mrna localization in rice endosperm cells[open] Plant Cell, 30(10), 2529-2552. https://doi.org/10.1105/tpc.18.00321

Rna-binding protein rbp-p is required for glutelin and prolamine mrna localization in rice endosperm cells[open] . / Tian, Li; Chou, Hong Li; Zhang, Laining; Hwang, Seon Kap; Starkenburg, Shawn R.; Doroshenk, Kelly A.; Kumamaru, Toshihiro; Okita, Thomas W.

In: Plant Cell, Vol. 30, No. 10, 01.01.2018, p. 2529-2552.

Research output: Contribution to journalArticle

Tian, L, Chou, HL, Zhang, L, Hwang, SK, Starkenburg, SR, Doroshenk, KA, Kumamaru, T & Okita, TW 2018, 'Rna-binding protein rbp-p is required for glutelin and prolamine mrna localization in rice endosperm cells[open] ', Plant Cell, vol. 30, no. 10, pp. 2529-2552. https://doi.org/10.1105/tpc.18.00321
Tian, Li ; Chou, Hong Li ; Zhang, Laining ; Hwang, Seon Kap ; Starkenburg, Shawn R. ; Doroshenk, Kelly A. ; Kumamaru, Toshihiro ; Okita, Thomas W. / Rna-binding protein rbp-p is required for glutelin and prolamine mrna localization in rice endosperm cells[open] In: Plant Cell. 2018 ; Vol. 30, No. 10. pp. 2529-2552.
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