RNA editing in gymnosperms and its impact on the evolution of the mitochondrial coxI gene

Meng Zhu Lu, Alfred Szmidt, Xiao Ru Wang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Sequence analysis of the mitochondrial coxI gene in eight gymnosperm species revealed a high rate of non-synonymous nucleotide substitutions with a strong (98%) predominance of C-T substitutions. Further analysis of the corresponding coxI cDNA sequences showed that all the non-synonymous C-T changes in the coxI genomic DNA sequences were eliminated by RNA editing resulting in nearly identical mRNA (amino acid) sequences among the species. Pronounced variation in the number and location of edited sites was found among species. Most species had a relatively large number of edited sites (from 25 to 34). However, no RNA editing of the coxI sequence was found in Gingko biloba or Larix sibirica. The sequence composition of the investigated coxI fragment suggests that the coxI gene in G. biloba and L. sibirica originated from edited mitochondrial coxI transcripts by reverse transcription followed by insertion into the nuclear genome or back into the mitochondrial genome. Our results also demonstrate that where there are a large number of edited sites, RNA editing can accelerate the divergence of nucleotide sequences among species.

Original languageEnglish
Pages (from-to)225-234
Number of pages10
JournalPlant Molecular Biology
Volume37
Issue number2
DOIs
Publication statusPublished - May 1 1998

Fingerprint

Gymnosperms
RNA Editing
RNA editing
Mitochondrial Genes
Larix sibirica
Ginkgo biloba
Larix
nucleotide sequences
Mitochondrial Genome
reverse transcription
genes
nuclear genome
Reverse Transcription
Sequence Analysis
Amino Acid Sequence
amino acid sequences
Nucleotides
sequence analysis
Complementary DNA
nucleotides

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

RNA editing in gymnosperms and its impact on the evolution of the mitochondrial coxI gene. / Lu, Meng Zhu; Szmidt, Alfred; Wang, Xiao Ru.

In: Plant Molecular Biology, Vol. 37, No. 2, 01.05.1998, p. 225-234.

Research output: Contribution to journalArticle

Lu, Meng Zhu ; Szmidt, Alfred ; Wang, Xiao Ru. / RNA editing in gymnosperms and its impact on the evolution of the mitochondrial coxI gene. In: Plant Molecular Biology. 1998 ; Vol. 37, No. 2. pp. 225-234.
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