RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.
|Number of pages||5|
|Journal||Journal of the Faculty of Agriculture, Kyushu University|
|Publication status||Published - Sep 2012|
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science