RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Jae Man Lee; Yoshito Kojin; Tsuneyuki Tatsuke; Hiroaki Mon; Yoshitaka Miyagawa; Takahiro Kusakabe

RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

Agricultural Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

Original language	English
Pages (from-to)	441-445
Number of pages	5
Journal	Journal of the Faculty of Agriculture, Kyushu University
Volume	57
Issue number	2
Publication status	Published - Sept 2012

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Biotechnology

Cite this

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title = "RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells",

abstract = "RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway{\textregistered}-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.",

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T1 - RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

AU - Lee, Jae Man

AU - Kojin, Yoshito

AU - Tatsuke, Tsuneyuki

AU - Mon, Hiroaki

AU - Miyagawa, Yoshitaka

AU - Kusakabe, Takahiro

PY - 2012/9

Y1 - 2012/9

N2 - RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

AB - RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

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M3 - Article

AN - SCOPUS:84866674786

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JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

IS - 2

ER -

RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Abstract

All Science Journal Classification (ASJC) codes

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