RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

Original languageEnglish
Pages (from-to)441-445
Number of pages5
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume57
Issue number2
Publication statusPublished - Sep 2012

Fingerprint

Bombyx
Bombyx mori
RNA Interference
RNA interference
Double-Stranded RNA
double-stranded RNA
DNA
cells
Reporter Genes
reporter genes
Genes
genes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Biotechnology

Cite this

RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells. / Lee, Jae Man; Kojin, Yoshito; Tatsuke, Tsuneyuki; Mon, Hiroaki; Miyagawa, Yoshitaka; Kusakabe, Takahiro.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 57, No. 2, 09.2012, p. 441-445.

Research output: Contribution to journalArticle

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