RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

Takeshi Hatta, Rika Umemiya, Min Liao, Haiyan Gong, Thasaneeya Harnnoi, Miho Tanaka, Takeharu Miyoshi, Damdinsuren Boldbaatar, Badgar Battsetseg, Jinlin Zhou, Xuenan Xuan, Naotoshi Tsuji, Demar Taylor, Kozo Fujisaki

Research output: Contribution to journalArticle

Abstract

Ticks are effective vectors of pathogens because of their blood feeding and high fecundity. This high fecundity is related to the size of the blood meal. Therefore, knowledge of how blood proteins are degraded and converted to proteins, including yolk protein, is important for the development of ways to inhibit the utilization of blood proteins by ticks. RNA interference (RNAi) is becoming a powerful post-transcriptional gene silencing technique that provides insight into gene function. We constructed a double-stranded RNA (dsRNA) based on a previously cloned Haemaphysalis longicornis leucine aminopeptidase (HlLAP) gene to reevaluate the biological role in tick blood digestion. Gene specific transcriptional, translational, and functional disruptions were achieved by the introduction of dsRNA into the ticks. Significantly delayed onset of egg-laying and reduced egg oviposition resulted from the RNAi for the HlLAP gene. These results suggest that HlLAP actually works as a blood digestive enzyme and affects tick fecundity via unknown mechanisms. The reduction of egg oviposition may be caused by a decrease in nutrients, especially free amino acids generated by HlLAP, from the blood meal. This is the first report of an impact on tick reproduction caused by gene silencing of a blood digestion-related molecule.

Original languageEnglish
Pages (from-to)847-54
Number of pages8
JournalParasitology Research
Volume100
Issue number4
DOIs
Publication statusPublished - Mar 2007

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Haemaphysalis longicornis
Ixodidae
Leucyl Aminopeptidase
leucyl aminopeptidase
Ticks
RNA Interference
RNA interference
Fertility
ticks
fecundity
Ovum
Oviposition
oviposition
Double-Stranded RNA
blood
blood meal
double-stranded RNA
Genes
blood proteins
Meals

Cite this

RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis. / Hatta, Takeshi; Umemiya, Rika; Liao, Min; Gong, Haiyan; Harnnoi, Thasaneeya; Tanaka, Miho; Miyoshi, Takeharu; Boldbaatar, Damdinsuren; Battsetseg, Badgar; Zhou, Jinlin; Xuan, Xuenan; Tsuji, Naotoshi; Taylor, Demar; Fujisaki, Kozo.

In: Parasitology Research, Vol. 100, No. 4, 03.2007, p. 847-54.

Research output: Contribution to journalArticle

Hatta, T, Umemiya, R, Liao, M, Gong, H, Harnnoi, T, Tanaka, M, Miyoshi, T, Boldbaatar, D, Battsetseg, B, Zhou, J, Xuan, X, Tsuji, N, Taylor, D & Fujisaki, K 2007, 'RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis', Parasitology Research, vol. 100, no. 4, pp. 847-54. https://doi.org/10.1007/s00436-006-0336-3
Hatta, Takeshi ; Umemiya, Rika ; Liao, Min ; Gong, Haiyan ; Harnnoi, Thasaneeya ; Tanaka, Miho ; Miyoshi, Takeharu ; Boldbaatar, Damdinsuren ; Battsetseg, Badgar ; Zhou, Jinlin ; Xuan, Xuenan ; Tsuji, Naotoshi ; Taylor, Demar ; Fujisaki, Kozo. / RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis. In: Parasitology Research. 2007 ; Vol. 100, No. 4. pp. 847-54.
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