RNA-primed PCR

Hiroki Shibata, Tomoko Tahira, Kenshi Hayashi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.

Original languageEnglish
Pages (from-to)400-403
Number of pages4
JournalGenome Research
Issue number4
Publication statusPublished - Nov 1995

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)


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