RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells

Ling Wei, Zhiqing Li, Daojun Cheng, Takahiro Kusakabe, Minhui Pan, Jun Duan, Yonghu Wang, Cheng Lu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The transcription factor SoxE is mainly expressed in the gonad and involved in the regulation of gonad development and sex determination in animals. Here, we used the silkworm ovary-derived BmN4-SID1 cell line to survey the roles of the silkworm SoxE protein (BmSoxE) and predict its candidate binding targets. RNAi-mediated silencing of BmSoxE expression suppressed cell proliferation in BmN4-SID1 cells. A further cell cycle analysis revealed that this inhibition of cell proliferation was largely due to cell cycle arrest in G1 phase when BmSoxE expression was blocked in BmN4-SID1 cells. Genome-wide microarray expression analyses demonstrated that the expression levels of a set of genes were significantly altered following BmSoxE RNAi. More than half of these genes contained conserved binding sites for HMG box domain of the Sox proteins and were predicted to be candidate binding targets for BmSoxE. Importantly, some of the candidate targets may be associated with the effect of BmSoxE on cell proliferation. Several candidate target genes showed gonad-specific expression in silkworm larvae. Taken together, these data demonstrate that BmSoxE is required for cell proliferation in silkworm BmN4-SID1 cells and provide valuable information for further investigations of the molecular control exerted by the BmSoxE protein over cell proliferation and gonad development in the silkworm.

Original languageEnglish
Pages (from-to)4769-4781
Number of pages13
JournalMolecular biology reports
Volume41
Issue number7
DOIs
Publication statusPublished - Jan 1 2014

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Bombyx
Gene Silencing
RNA Interference
Gonads
Cell Proliferation
HMG-Box Domains
Genes
Sexual Development
Proteins
G1 Phase
Microarray Analysis
Cell Cycle Checkpoints
Larva
Ovary
Cell Cycle
Transcription Factors
Binding Sites
Genome
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells. / Wei, Ling; Li, Zhiqing; Cheng, Daojun; Kusakabe, Takahiro; Pan, Minhui; Duan, Jun; Wang, Yonghu; Lu, Cheng.

In: Molecular biology reports, Vol. 41, No. 7, 01.01.2014, p. 4769-4781.

Research output: Contribution to journalArticle

Wei, Ling ; Li, Zhiqing ; Cheng, Daojun ; Kusakabe, Takahiro ; Pan, Minhui ; Duan, Jun ; Wang, Yonghu ; Lu, Cheng. / RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells. In: Molecular biology reports. 2014 ; Vol. 41, No. 7. pp. 4769-4781.
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