Rod-like architecture and helicity of the poly(C)/schizophyllan complex observed by AFM and SEM

Ah Hyun Bae, Seung Woo Lee, Masato Ikeda, Masahito Sano, Seiji Shinkai, Kazuo Sakurai

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Microscopic studies of the complex between poly(C) and schizophyllan (SPG), employing both AFM and SEM, revealed that the complex takes the same rod-like architecture on the mica surface as those of the renatured SPG and the original triple helix of SPG, indicating that the complex also has a helical structure. The SEM observations showed the helical pattern on the rod surface, only when the sample was metal shadowed. The pitch evaluated from the image is comparable with that obtained from crystallographic data. The ability to visualize the helical structure can be explained from the hypothesis that the platinum grains may assemble on the sample using the molecular surface of the SPG (or complex) as the template.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalCarbohydrate Research
Volume339
Issue number2
DOIs
Publication statusPublished - Jan 22 2004

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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