Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2)

Daisuke Asai, Masaharu Murata, Riki Toita, Takahito Kawano, Hideki Nakashima, Jeong Hun Kang

    Research output: Contribution to journal › Article

    2 Citations (Scopus)

    Abstract

    A series of amino acid substitutions was made in a previously identified β-tubulin-derived GRK2 substrate peptide (404DEMEFTEAESNMN416) to examine the role of amino acid residues surrounding the phosphorylation site. Anionic amino acid residues surrounding the phosphorylation site played an important role in the affinity for GRK2. Compared to the original peptide, a modified peptide (Ac-EEMEFSEAEANMN-NH2) exhibited markedly higher affinity for GRK2, but very low affinity for GRK5, suggesting that it can be a sensitive and selective peptide for GRK2.

    Original languageEnglish
    Pages (from-to)2875-2880
    Number of pages6
    JournalAmino Acids
    Volume48
    Issue number12
    DOIs
    Publication statusPublished - Dec 1 2016

    Fingerprint

    G-Protein-Coupled Receptor Kinase 2
    Phosphorylation
    Amino Acids
    Peptides
    Substrates
    Amino Acid Substitution
    Tubulin
    Substitution reactions

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Clinical Biochemistry
    • Organic Chemistry

    Cite this

    Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2). / Asai, Daisuke; Murata, Masaharu; Toita, Riki; Kawano, Takahito; Nakashima, Hideki; Kang, Jeong Hun.

    In: Amino Acids, Vol. 48, No. 12, 01.12.2016, p. 2875-2880.

    Research output: Contribution to journal › Article

    Asai, D, Murata, M, Toita, R, Kawano, T, Nakashima, H & Kang, JH 2016, 'Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2)', Amino Acids, vol. 48, no. 12, pp. 2875-2880. https://doi.org/10.1007/s00726-016-2345-6
    Asai D, Murata M, Toita R, Kawano T, Nakashima H, Kang JH. Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2). Amino Acids. 2016 Dec 1;48(12):2875-2880. https://doi.org/10.1007/s00726-016-2345-6
    Asai, Daisuke ; Murata, Masaharu ; Toita, Riki ; Kawano, Takahito ; Nakashima, Hideki ; Kang, Jeong Hun. / Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2). In: Amino Acids. 2016 ; Vol. 48, No. 12. pp. 2875-2880.
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    AU - Toita, Riki

    AU - Kawano, Takahito

    AU - Nakashima, Hideki

    AU - Kang, Jeong Hun

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