Role of c-jun in the inhibition of erythropoietin receptor-mediated apoptosis

Ritsuko Shimizu, Norio Komatsu, Yukio Nakamura, Hiromitsu Nakauchi, Yusaku Nakabeppu, Yasusada Miura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Human bone marrow cells express both a truncated and full-length form of the erythropoietin receptor (EpoR-T and EpoR-F, respectively). Transfection experiments using the murine interleukin (IL)-3-dependent cell line, Ba/F3, revealed that the cells coexpressing EpoR-F and EpoR-T (Ba/F3-FT) were more likely to undergo programmed cell death (apoptosis) than cells expressing EpoR-F (Ba/F3-FF), even in the presence of erythropoietin (Epo). When Ba/F3-FF cells were stimulated with Epo or IL-3, rapid induction of c-myc, c-fos, c-jun and junB genes was observed. A similar effect was also seen in IL-3-stimulated Ba/F3-Ft cells. However, in Ba/F3-FT cells expression of the c-jun gene was not induced by Epo stimulation. Administration of Epo could prevent apoptosis induced by IL-3 deprivation in Ba/F3-FT cells expressing ectopic c-Jun protein. These results indicate that induction of c-Jun through the Epo signaling pathway has an important role in the inhibition of apoptosis.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume222
Issue number1
DOIs
Publication statusPublished - May 6 1996

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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