Role of confinement in the active self-organization of kinesin-driven microtubules

Md Sirajul Islam, Kaori Kuribayashi-Shigetomi, Arif Md Rashedul Kabir, Daisuke Inoue, Kazuki Sada, Akira Kakugo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Self-organization is one of the most spectacular phenomena exhibited in the wide spectrum of biologically active systems. Many studies have attempted to investigate different parameters that regulate the self-organization of moving objects. Recent theoretical and analytical-based approaches have revealed that physical confinement has regulatory effect on the self-organization of moving objects. However, a detailed experimental study on how the varying shapes and sizes of the confinement affect the self-organization of moving objects is still lacking. Recently, biomolecular motor systems F-actin/myosin and microtubule/kinesin or microtubule/dynein have been promising to experimentally study the self-organization of moving objects. Here, we experimentally investigated the shape and size effect of confinement on the self-organization of microtubules (MTs) by employing the in vitro motility assay of MT/kinesin motor system. The MTs were confined by a lipid layer on a glass surface micro-patterned by photolithography. We demonstrated that shapes and sizes of the confinements largely influenced the self-organization of MTs. The MTs showed distinct orientations in different shapes and sizes of the confinements. This work clearly unveiled how physical confinement influences the self-organization of MTs and would help understand the effect of confinement on the self-organization of more complex biologically active systems in nature.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume247
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

Fingerprint

Kinesin
efferent nervous systems
myosins
locomotion
photolithography
Dyneins
lipids
Photolithography
Myosins
Lipids
Actins
Assays
glass
Glass

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Role of confinement in the active self-organization of kinesin-driven microtubules. / Islam, Md Sirajul; Kuribayashi-Shigetomi, Kaori; Kabir, Arif Md Rashedul; Inoue, Daisuke; Sada, Kazuki; Kakugo, Akira.

In: Sensors and Actuators, B: Chemical, Vol. 247, 01.01.2017, p. 53-60.

Research output: Contribution to journalArticle

Islam, Md Sirajul ; Kuribayashi-Shigetomi, Kaori ; Kabir, Arif Md Rashedul ; Inoue, Daisuke ; Sada, Kazuki ; Kakugo, Akira. / Role of confinement in the active self-organization of kinesin-driven microtubules. In: Sensors and Actuators, B: Chemical. 2017 ; Vol. 247. pp. 53-60.
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