Role of iron in oxidative stress in skeletal muscle atrophied by immobilization

H Kondo, M Miura, J Kodama, S M Ahmed, Y Itokawa

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

To clarify the role of iron in oxidative stress in skeletal muscle atrophied by immobilization, we investigated the effect of deferoxamine--an iron-chelating agent. Deferoxamine, iron-saturated deferoxamine and double-distilled water (control) were administered subcutaneously from the 4th day after immobilization via osmotic pumps to male Wistar rats (14 weeks old), one ankle joint of which was immobilized in the extended position. After 12 days' immobilization, soleus--typical slow red muscles were collected from both hind limbs and their levels of thiobarbituric acid-reactive substance (TBARS) and glutathione were measured. Deferoxamine suppressed the increase of TBARS and glutathione disulfide in atrophied muscle while iron-saturated deferoxamine did not, which strongly suggests that the iron-chelating action of deferoxamine suppressed the increased oxidative stress. This means that iron plays a very important role in increasing oxidative stress in atrophied muscle. In addition, deferoxamine decreased the degree of atrophy, an effect thought to be mediated by the suppression of oxidative stress.

Original languageEnglish
Pages (from-to)295-7
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume421
Issue number2-3
Publication statusPublished - Jun 1992

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Deferoxamine
Oxidative stress
Immobilization
Muscle
Skeletal Muscle
Oxidative Stress
Iron
Thiobarbituric Acid Reactive Substances
Muscles
Iron Chelating Agents
Ankle Joint
Glutathione Disulfide
Chelation
Atrophy
Glutathione
Wistar Rats
Rats
Extremities
Pumps
Water

Cite this

Role of iron in oxidative stress in skeletal muscle atrophied by immobilization. / Kondo, H; Miura, M; Kodama, J; Ahmed, S M; Itokawa, Y.

In: Pflugers Archiv European Journal of Physiology, Vol. 421, No. 2-3, 06.1992, p. 295-7.

Research output: Contribution to journalArticle

Kondo, H ; Miura, M ; Kodama, J ; Ahmed, S M ; Itokawa, Y. / Role of iron in oxidative stress in skeletal muscle atrophied by immobilization. In: Pflugers Archiv European Journal of Physiology. 1992 ; Vol. 421, No. 2-3. pp. 295-7.
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