Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness

Minoru Sawaguchi, Shinya Tanaka, Yuriko Nakatani, Yasuyo Harada, Kaori Mukai, Yuko Matsunaga, Kenji Ishiwata, Keisuke Oboki, Taku Kambayashi, Naohiro Watanabe, Hajime Karasuyama, Susumu Nakae, Hiromasa Inoue, Masato Kubo

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Abstract

We established a diphtheria toxin (DT)-based conditional deletion system using Il4 enhancer elements previously shown to be specific for IL-4 production in mast cells (MCs) or basophils (Mas-TRECK and Bas-TRECK mice). DT treatment of Bas-TRECK mice resulted in specific deletion of basophils, whereas both MCs and basophils were deleted in Mas-TRECK mice. DT-treated Mas-TRECK mice had impaired passive cutaneous anaphylaxis, IgE-mediated passive systemic anaphylaxis, and IgE-mediated chronic allergic inflammation, whereas DT-treated Bas-TRECK mice had impaired IgE-mediated chronic allergic inflammation. Using these mice, we also sought to tease out the role of MCs and basophils in airway hyperresponsiveness (AHR). Although MC deletion resulted in a slight increase in basal Ag-specific IgE levels and significant increases in basal IgE levels, we found that this deletion markedly impaired the AHR effector phase and was accompanied by decreased histamine levels. By contrast, basophil deletion had no effect on the AHR effector phase or on IgE production induced by systemic OVA immunization. Our results, using these newly established Mas-TRECK and Bas-TRECK models, demonstrated an indispensable role for MCs as effector cells in AHR.

Original languageEnglish
Pages (from-to)1809-1818
Number of pages10
JournalJournal of Immunology
Volume188
Issue number4
DOIs
Publication statusPublished - Feb 15 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Sawaguchi, M., Tanaka, S., Nakatani, Y., Harada, Y., Mukai, K., Matsunaga, Y., ... Kubo, M. (2012). Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness. Journal of Immunology, 188(4), 1809-1818. https://doi.org/10.4049/jimmunol.1101746